Silkworm pathogenic bacteria infection model for identification of novel virulence genes
Silkworm pathogenic bacteria infection model for identification of novel virulence genes
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DOI：
10.1111/j.1365-2958.2005.04596.x
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发表时间：
2005-05-01
影响因子：
3.6
通讯作者：
Sekimizu, K
中科院分区：
文献类型：
--
作者：
Kaito, C;Kurokawa, K;Sekimizu, K
将金黄色葡萄球菌和化脓性链球菌等病原菌注入血淋巴，可杀死家蚕。对金黄色葡萄球菌的基因中断突变体进行了家蚕毒力检测，发现金黄色葡萄球菌的开放阅读框尚未确定，且在细菌中保守。在这100个基因中，3个基因cvfA、cvfB和cvfc是金黄色葡萄球菌对家蚕的完全毒力所必需的。在这些突变体中，溶血素的产量降低。CvfA和cvfc突变体也降低了小鼠的毒力。化脓性链球菌cvfA破坏的突变体产生的外毒素较少，对家蚕和小鼠的毒力都有所减弱。这些结果表明，家蚕感染模型可用于鉴定细菌毒力基因。
Silkworms are killed by injection of pathogenic bacteria, such as Staphylococcus aureus and Streptococcus pyogenes, into the haemolymph. Gene disruption mutants of S. aureus whose open reading frames were previously uncharacterized and that are conserved among bacteria were examined for their virulence in silkworms. Of these 100 genes, three genes named cvfA, cvfB, and cvfC were required for full virulence of S. aureus in silkworms. Haemolysin production was decreased in these mutants. The cvfA and cvfC mutants also had attenuated virulence in mice. S. pyogenes cvfA-disrupted mutants produced less exotoxin and had attenuated virulence in both silkworms and mice. These results indicate that the silkworm-infection model is useful for identifying bacterial virulence genes.