UV-induced DNA damage and melanin content in human skin differing in racial/ethnic origin
UV-induced DNA damage and melanin content in human skin differing in racial/ethnic origin
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DOI：
10.1096/fj.02-0865fje
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发表时间：
2003-04-01
期刊：
影响因子：
4.8
通讯作者：
Hearing, VJ
中科院分区：
文献类型：
--
作者：
Tadokoro, T;Kobayashi, N;Hearing, VJ
紫外线辐射引起的DNA损伤是皮肤光致癌过程中的一个重要事件。然而，种族/民族血统在决定个体紫外线敏感性方面的作用仍不清楚。在这项研究中，我们研究了黑色素含量与不同种族/民族、光型和紫外线敏感性的正常皮肤原位紫外线照射引起的DNA损伤之间的关系。为每位暴露于UVA/UVB辐射的受试者确定最小红斑剂量（MED），并在暴露于UVA/UVB辐射前、7分钟、1天和1周分别对皮肤进行活检。个体之间在DNA损伤的数量和去除速度上有很大的差异。结果表明，在1 MED的紫外线照射下，各组受试者皮肤均出现明显的DNA损伤，且组成黑色素含量的增加与DNA损伤程度呈负相关。从这些结果中可以清楚地看出，测量皮肤红斑紫外线敏感性（MED）比种族/民族血统或皮肤光型更能预测DNA光损伤，紫外线辐射后DNA损伤去除率可能是皮肤紫外线敏感性（包括癌症易感性）的关键决定因素。
DNA damage induced by UV radiation is a critical event in skin photocarcinogenesis. However, the role of racial/ethnic origin in determining individual UV sensitivity remains unclear. In this study, we examined the relationships between melanin content and DNA damage induced by UV exposure in situ in normal human skin of different racial/ethnic groups, phototypes, and UV sensitivities. The minimal erythema dose (MED) was established for each subject exposed to UVA/UVB radiation, and skin was biopsied before as well as 7 min, 1 day, and 1 wk after UV exposure. There was great variation among individuals in the amount of DNA damage incurred and rates of its removal. The results show that after exposure to 1 MED of UV, the skin of subjects from all groups suffered significant DNA damage, and that increasing content of constitutive melanin inversely correlated with the amount of DNA damage. It is clear from these results that measured erythemal UV sensitivity of the skin (MED) is a more useful predictor of DNA photodamage than is racial/ethnic origin or skin phototype and that rates of DNA damage removal following UV radiation may be the critical determinant of the UV sensitivity (including predisposition to cancer) of the skin.