Gustducin is expressed in the taste buds of the chicken
Gustducin is expressed in the taste buds of the chicken
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DOI：
10.1111/j.1740-0929.2010.00796.x
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发表时间：
2010-01-01
影响因子：
2
通讯作者：
Tabata, Shoji
中科院分区：
文献类型：
--
作者：
Kudo, Ken-ichi;Wakamatsu, Kei-ichi;Tabata, Shoji
本研究采用分子生物学、生物化学和免疫组织化学技术研究了味觉调节素在鸡味蕾中的表达。通过RT-PCR检测到一个味蛋白样序列在鸡味蕾组织中的表达，与预测的鸡味蛋白基因相对应。该序列在鸡的脑、心、肝、胰、肠、肾和睾丸中均未检测到表达。表达序列对含有味蕾的口腔组织具有高度特异性。这些结果表明，检测到的序列是鸡gustducin基因。接下来，我们产生了一个多克隆抗血清对鸡gustducin蛋白，以观察其在口腔组织中的定位。结果表明，鸡味素在味蕾中特异性表达。提示鸡具有由味蛋白介导的味觉系统，鸡味蛋白是味蕾或味细胞的可靠标记物。这是第一个分子生物学，生物化学和免疫组织化学证明的存在gustducin在鸡。
We investigated the expression of gustducin in chicken taste buds using molecular biological, biochemical and immunohistochemical techniques. Expression of a gustducin-like sequence was detected by RT-PCR in the tissues containing taste buds, and corresponded to the predicted gustducin gene in the chicken. Expression of this sequence was not detected in the brain, heart, liver, pancreas, intestine, kidney and testis of the chicken. The expressed sequence had a high specificity for oral tissues that contained taste buds. These results suggest that the detected sequence was the chicken gustducin gene. Next, we generated a polyclonal antiserum against the chicken gustducin protein to observe its localization in the oral tissues. The results revealed that the chicken gustducin was specifically expressed in the taste buds. It is suggested that the chicken has a gustatory system mediated by gustducin, and chicken gustducin is a reliable marker for taste buds or taste cells. This is the first molecular biological, biochemical and immunohistochemical demonstration of the presence of gustducin in the chicken.