Protocol for the MPTP mouse model of Parkinson's disease
Protocol for the MPTP mouse model of Parkinson's disease
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DOI：
10.1038/nprot.2006.342
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Przedborski, Serge
中科院分区：
文献类型：
--
作者：
Jackson-Lewis, Vernice;Przedborski, Serge
本方案描述了我们使用神经毒素1-甲基-4-苯基-1，2，3，6-四氢吡啶(MPTP)建立可靠的帕金森病(PD)小鼠模型的方法。我们讨论了模型的细节，提供了关键参考，并概述了研究人员需要了解的内容，以安全和成功地建立MPTP小鼠帕金森病模型。该议定书的完成取决于所使用的MPTP方案和实际计划的研究，通常从7至30天不等。该议定书要求实施安全措施并购置几件设备，如果选择定期使用这一模式，这是值得进行的一次性投资。
This protocol describes our method of producing a reliable mouse model of Parkinson's disease (PD) using the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). We discuss the particulars of the model, provide key references and outline what investigators need to know to develop the MPTP mouse model of PD safely and successfully. Completion of this protocol depends on the regimen of MPTP used and on the actual planned studies, which often range from 7 to 30 d. This protocol calls for implementation of safety measures and for the acquisition of several pieces of equipment, which are a one-time investment worth making if one elects to use this model on a regular basis.