Dietary inclusion of colicin E1 is effective in preventing postweaning diarrhea caused by F18-positive Escherichia coli in pigs
Dietary inclusion of colicin E1 is effective in preventing postweaning diarrhea caused by F18-positive Escherichia coli in pigs
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DOI：
10.1128/aac.00360-07
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发表时间：
2007-11-01
影响因子：
4.9
通讯作者：
Stahl, C. H.
中科院分区：
文献类型：
--
作者：
Cutler, S. A.;Lonergan, S. M.;Stahl, C. H.
随着全世界对畜牧业中抗生素的使用及其对抗生素耐药性传播的贡献的关注，需要常规抗生素的替代品。我们实验室先前的研究表明，大肠杆菌素El在体外对一些引起断奶后腹泻（PWD）的大肠杆菌菌株有效。在这项研究中，我们检查了大肠杆菌素E1在预防由F18阳性肠球菌性大肠杆菌引起的实验诱导的PWD中的功效。仔猪大肠杆菌。24头断奶仔猪（23日龄），经基因分型鉴定为对F18阳性E.大肠杆菌感染，单独圈养，并饲喂含有0，11，或16.5毫克大肠杆菌素E1/公斤饮食。试验开始后两天，所有动物经口接种两株F18阳性E.分离自PWD猪的大肠杆菌菌株。大肠杆菌素E1可降低F18阳性产肠球菌引起的PWD的发生率和严重程度。提高仔猪的生长性能。此外，由于饮食大肠杆菌素E1导致的PWD发病率降低，降低了这些动物回肠组织中白细胞介素1 β和肿瘤坏死因子β基因的表达水平。在断奶仔猪日粮中添加大肠杆菌素E1可有效地替代常规抗生素，预防F18阳性肠出血性大肠杆菌引起的PWD。杆菌
With worldwide concern over the use of antibiotics in animal agriculture and their contribution to the spread of antibiotic resistance, alternatives to conventional antibiotics are needed. Previous research in our laboratories has shown that colicin El is effective against some Escherichia coli strains responsible for postweaning diarrhea (PWD) in vitro. In this study we examined the efficacy of the dietary inclusion of colicin El in preventing experimentally induced PWD caused by F18-positive enterotoxigenic E. coli in young pigs. Twenty-four weaned pigs (23 days of age), identified by genotyping to be susceptible to F18-positive E. coli infections, were individually housed and fed diets containing 0, 11, or 16.5 mg colicin E1/kg diet. Two days after the start of the trial, all animals were orally inoculated with 1 X 10(9) CFU of each of two F18-positive E. coli strains isolated from pigs with PWD. The dietary inclusion of colicin El decreased the incidence and severity of PWD caused by F18-positive enterotoxigenic E. coli and improved the growth performance of the piglets. Additionally, the reduced incidence of PWD due to dietary colicin El, lowered the levels of expression of the genes for interleukin 1 beta and tumor necrosis factor beta in ileal tissues from these animals. The dietary inclusion of colicin El may be an effective alternative to conventional antibiotics in the diets of weaning pigs for the prevention of PWD caused by F18-positive enterotoxigenic E. coli.