A CIS-ACTING ELEMENT IS NECESSARY AND SUFFICIENT FOR TRANSLATIONAL REGULATION OF HUMAN FERRITIN EXPRESSION IN RESPONSE TO IRON
A CIS-ACTING ELEMENT IS NECESSARY AND SUFFICIENT FOR TRANSLATIONAL REGULATION OF HUMAN FERRITIN EXPRESSION IN RESPONSE TO IRON
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DOI：
10.1073/pnas.84.19.6730
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发表时间：
1987-10-01
影响因子：
11.1
通讯作者：
KLAUSNER, RD
中科院分区：
文献类型：
--
作者：
HENTZE, MW;ROUAULT, TA;KLAUSNER, RD
铁蛋白在决定铁在细胞内的命运中起着关键作用，并受到细胞铁状态的高度调控。我们在转录的但未翻译的人铁蛋白重链mRNA的5‘’前导序列中发现了一个顺式作用元件。在瞬时转基因的小鼠成纤维细胞中，5‘’前导序列的157个核苷酸区域的存在被发现是铁蛋白生物合成的铁依赖调节所必需的。此外，这个5‘’前导区足以将铁介导的翻译控制转移到异源基因产物氯霉素乙酰转移酶的表达上。
Ferritin plays a key role in determining the intracellular fate of iron and is highly regulated by the iron status of the cell. We have identified a cis-acting element in the transcribed but nontranslated 5'' leader sequence of human ferritin heavy-chain mRNA. In transiently transfected murine fibroblasts, the presence of a 157-nucleotide region of the 5'' leader sequence was found to be necessary for iron-dependent regulation of ferritin biosynthesis. Further, this 5'' leader region is sufficient to transfer iron-mediated translational control to the expression of a heterologous gene product, chloramphenicol acetyltransferase.