Ellagic acid, phenolic acids, and flavonoids in Malaysian honey extracts demonstrate in vitro anti-inflammatory activity
Ellagic acid, phenolic acids, and flavonoids in Malaysian honey extracts demonstrate in vitro anti-inflammatory activity
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DOI：
10.1016/j.nutres.2010.08.008
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发表时间：
2010-09-01
影响因子：
4.5
通讯作者：
Yusoff, Kamaruddin Mohd
中科院分区：
文献类型：
--
作者：
Kassim, Mustafa;Achoui, Mouna;Yusoff, Kamaruddin Mohd
天然蜂蜜在世界各地不同文化的传统医学中都有应用。本研究对马来西亚蜂蜜的提取以及这些提取物的抗炎活性进行了评估。据推测，蜂蜜提取物含有不同量的酚类化合物，并且它们具有不同的体外抗炎活性。使用液相色谱 - 质谱法对蜂蜜提取物进行分析以识别和比较酚类化合物，而高效液相色谱法则用于对其进行定量。随后，在体外测试了蜂蜜甲醇提取物（HME）和蜂蜜乙酸乙酯提取物（HEAE）对受刺激的巨噬细胞中一氧化氮产生的影响。还测试了这些提取物对L929细胞中肿瘤坏死因子 -α（TNF）细胞毒性的影响。提取物中的主要酚类物质是鞣花酸、没食子酸和阿魏酸；杨梅素；绿原酸和咖啡酸。其他浓度较低的化合物有橙皮素、对香豆酸、白杨素、槲皮素、木犀草素和山奈酚。鞣花酸是所记录的酚类化合物中含量最丰富的，在HME和HEAE中，其平均浓度分别为每100克蜂蜜3295.83微克和626.74微克。HEAE和HME对体外一氧化氮抑制的中位最大有效浓度分别计算为37.5微克/毫升和271.7微克/毫升。HEAE和HME对TNF细胞毒性保护的中位最大有效浓度分别为168.1微克/毫升和235.4微克/毫升。总之，HEAE在体外表现出更强的活性，而HME每100克蜂蜜中含有更高浓度的酚类化合物。（C）2010爱思唯尔公司。保留所有权利。
Natural honey has been used in traditional medicine of different cultures throughout the world. This study looked into the extraction of Malaysian honey and the evaluation of the anti-inflammatory activity of these extracts. It was hypothesized that honey extracts contain varying amounts of phenolic compounds and that they possess different in vitro anti-inflammatory activities. Honey extracts were analyzed using liquid chromatography-mass spectrometry to identify and compare phenolic compounds, whereas high-performance liquid chromatography was used for their quantification. Subsequently, honey methanol extract (HME) and honey ethyl acetate extract (HEAE) were tested in vitro for their effect on nitric oxide production in stimulated macrophages. The extracts were also tested for their effects on tumor necrosis factor-alpha (TNF) cytotoxicity in L929 cells. The major phenolics in the extracts were ellagic, gallic, and ferulic acids; myricetin; chlorogenic acid; and caffeic acid. Other compounds found in lower concentrations were hesperetin, p-coumaric acid, chrysin, quercetin, luteolin, and kaempferol. Ellagic acid was the most abundant of the phenolic compounds recorded, with mean concentrations of 3295.83 and 626.74 mu g/100 g of honey in HME and HEAE, respectively. The median maximal effective concentrations for in vitro nitric oxide inhibition by HEAE and HME were calculated to be 37.5 and 271.7 mu g/mL, respectively. The median maximal effective concentrations for protection from TNF cytotoxicity by HEAE and HME were 168.1 and 235.4 mu g/mL, respectively. In conclusion, HEAE exhibited greater activity in vitro, whereas HME contained a higher concentration of phenolic compounds per 100 g of honey. (C) 2010 Elsevier Inc. All rights reserved.