Procyanidins Modulate MicroRNA Expression in Pancreatic Islets
Procyanidins Modulate MicroRNA Expression in Pancreatic Islets
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DOI：
10.1021/jf303972f
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发表时间：
2013-01-16
影响因子：
6.1
通讯作者：
Ardevol, Anna
中科院分区：
文献类型：
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作者：
Castell-Auvi, Anna;Cedo, Lidia;Ardevol, Anna
原花青素调节葡萄糖代谢，部分原因是其对胰腺的影响。鉴于microRNAs（miRNAs）在糖尿病调控中的作用以及类黄酮调节其他组织中miRNAs的事实，我们假设原花青素可能靶向胰腺中的miRNAs。我们研究了从每日接受葡萄籽原花青素提取物（GSPE）（25 mg/kg体重）治疗45天的大鼠中分离的胰岛中的miRNA表达谱。miRWalk数据库鉴定了这些miRNA的推定靶基因。我们发现GSPE显著改变了miR-1249、miR-483、miR-30 c-1* 和miR-3544的表达。计算机模拟预测研究表明，离子转运和对葡萄糖的反应是受调节的途径之一。总之，这是第一个研究表明，原花青素也可以发挥其生物活性的胰岛通过修改的miRNA表达模式。
Procyanidins modulate glucose metabolism, partly due to its effects on pancreas. Given the role of microRNAs (miRNAs) in the regulation of diabetes and the fact that flavonoids modulate miRNAs in other tissues, we hypothesized that procyanidins might target miRNAs in the pancreas. We investigated the miRNA expression profile in pancreatic islets isolated from rats treated with a daily dose of grape seed procyanidin extract (GSPE) (25 mg/kg of body weight) for 45 days. The miRWalk database identified putative target genes of these miRNAs. We found that GSPE altered significantly the expression of miR-1249, miR-483, miR-30c-1*, and miR-3544. In silico prediction studies suggested that ion transport and response to glucose are among the regulated pathways. As a conclusion, this is the first study showing that procyanidins can also exert their bioactivity on pancreatic islets by modifying the miRNA expression pattern.