Molecular Evidence for Multiple Infections as Revealed by Typing of Asaia Bacterial Symbionts of Four Mosquito Species
Molecular Evidence for Multiple Infections as Revealed by Typing of Asaia Bacterial Symbionts of Four Mosquito Species
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DOI：
10.1128/aem.01747-10
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发表时间：
2010-11-01
影响因子：
4.4
通讯作者：
Bandi, Claudio
中科院分区：
文献类型：
--
作者：
Chouaia, Bessem;Rossi, Paolo;Bandi, Claudio
最近增加的检测Asaia属的乙酸细菌（AAB）作为蚊子，如按蚊属的共生体。和伊蚊属，促使我们研究这些共生体的多样性及其在不同蚊子物种和种群中的关系。在依赖培养和不依赖培养的技术之后，我们调查了与四种蚊子物种，斯氏按蚊、冈比亚按蚊、埃及伊蚊和白纹伊蚊相关的微生物群，这四种蚊子是人类和/或动物病原体的重要载体。变性梯度凝胶电泳（DGGE）分析的基础上的16 S rRNA基因揭示了几个细菌类群的存在，其中Asaia序列在大多数样品中占主导地位。收集了281 Asaia菌株在无细胞培养基中建立了属于四个物种的个人。通过ITS-PCR、tRNA-PCR、BOX-PCR和RAPD-PCR对分离株进行了分型，结果表明不同的Asaia菌株存在于不同的蚊群中，甚至存在于同一个体中。
The recent increased detection of acetic acid bacteria (AAB) of the genus Asaia as symbionts of mosquitoes, such as Anopheles spp. and Aedes spp., prompted us to investigate the diversity of these symbionts and their relationships in different mosquito species and populations. Following cultivation-dependent and -independent techniques, we investigated the microbiota associated with four mosquito species, Anopheles stephensi, Anopheles gambiae, Aedes aegypti, and Aedes albopictus, which are important vectors of human and/or animal pathogens. Denaturing gradient gel electrophoresis (DGGE) analysis based on the 16S rRNA gene revealed the presence of several bacterial taxa, among which Asaia sequences were among the dominant in most of the samples. A collection of 281 Asaia isolates in cell-free media was established from individuals belonging to the four species. The isolates were typed by internal transcribed spacer (ITS)-PCR, tRNA-PCR, BOX-PCR, and randomly amplified polymorphic DNA (RAPD)-PCR, revealing that different Asaia strains are present in different mosquito populations, and even in single individuals.