Side effects of control treatment can conceal experimental data when studying stress responses to injection and psychological stress in mice
Side effects of control treatment can conceal experimental data when studying stress responses to injection and psychological stress in mice
复制标题
DOI：
10.1038/laban0411-119
复制
发表时间：
2011-04-01
期刊：
影响因子：
6.9
通讯作者：
Kiank-Nussbaum, Cornelia
中科院分区：
文献类型：
--
作者：
Drude, Solveig;Geissler, Annett;Kiank-Nussbaum, Cornelia
常规实验室程序，如处理或运输动物或对动物进行注射，对动物来说是有压力的，但在许多临床前研究中是必要的。在本文中，作者表明多次注射无毒溶剂环糊精适度增加了雌性BALB/c小鼠的血浆皮质酮浓度。此外，接受单次腹膜内注射无害盐水的雄性BALB/c小鼠对第二次盐水注射的糖皮质激素反应增加。作者发现，暴露于急性心理应激期的雌性小鼠对第二种同型应激源的糖皮质激素反应降低。相比之下，多次心理应激导致雌性小鼠糖皮质激素释放增加。雄性小鼠急性注射生理盐水和雌性小鼠急性注射环糊精导致一过性淋巴细胞减少症。进一步分析表明，反复应激诱导的淋巴细胞减少是糖皮质激素依赖性的。作者得出结论，实验室压力可以影响小鼠的生理参数，可能会改变研究结果。
Routine laboratory procedures, such as handling or transporting animals or carrying out injections on animals, are stressful for animals but are necessary in many preclinical studies. Here, the authors show that multiple injections of the non-toxic vehicle cyclodextrin moderately increased plasma corticosterone concentrations in female BALB/c mice. Additionally, male BALB/c mice that had received a single intraperitoneal injection of harmless saline had an increased glucocorticoid response to a second saline injection. The authors found that female mice that had been exposed to an acute psychological stress session had a decreased glucocorticoid response to a second homotypic stressor. In contrast, multiple psychological stress sessions led to increased glucocorticoid release in female mice. Acute injection(s) of saline in male mice and of cyclodextrin in female mice led to transient lymphocytopenia. Further analysis showed that repeated stress induced lymphocytopenia is glucocorticoid-dependent. The authors conclude that laboratory stress can affect physiological parameters in mice, potentially altering study results.