ESTABLISHMENT OF A RABBIT SHORT-TERM DRY EYE MODEL
ESTABLISHMENT OF A RABBIT SHORT-TERM DRY EYE MODEL
复制标题
DOI：
10.1089/jop.1995.11.503
复制
发表时间：
1995-12-01
影响因子：
2.3
通讯作者：
SHIRASAWA, E
中科院分区：
文献类型：
--
作者：
FUJIHARA, T;NAGANO, T;SHIRASAWA, E
干眼症是一种慢性疾病，可能会对有效视力构成严重威胁。然而，对于这种疾病的有效治疗或预防，人们的了解还很有限。建立一种有效的治疗模式需要使用一种令人满意的动物干眼症模型。理想情况下，这种模型应能快速确定抑制该综合征有害影响的药物的有效性。本文介绍了一种使用兔子的短期干眼症模型，它结合了机械性阻止眨眼和亚甲蓝染色。这种模型并不旨在精确模拟干眼症综合征，因为人们最近认识到这种疾病涉及角膜和结膜上皮的原发性病理过程。然而，通过使用这种模型，干眼症的临床症状可在数小时后以急性干燥的形式被观察到。角膜损伤可以通过亚甲蓝染色评分进行定性评估，也可以通过长期检测进行定量评估。通过扫描电子显微镜（SEM）证实，肉眼观察到的角膜上皮变薄是由于上皮完整性的丧失。使用3%硫酸软骨素溶液（一种已被证明对干眼症有效的药物），该模型在仅2小时后就有效地显示出对亚甲蓝阳性病变发展的80%抑制作用。这种短期干眼症模型在初步筛选潜在治疗药物在干眼症预防和治疗中的功效方面具有重要价值。
The dry eye syndrome is a chronic disease which can become a serious threat to useful vision. However, there is only a limited understanding regarding effective treatment or prevention of this disease. Establishing an effective mode of treatment requires the use of a satisfactory animal dry eye model. Ideally, such a model should rapidly determine the effectiveness of agents that inhibit the damaging effects of this syndrome.This paper presents a short-term dry eye model using rabbits, which combines mechanical prevention of blinking and methylene blue staining. This model is not intended to be a precise representation of the dry eye syndrome, since this disorder has recently become recognized to involve a primary pathological process of the corneal and conjunctival epithelium. However, by using this model, clinical signs of dry eye can be observed after a few hours in the form of acute desiccation. Corneal damage can easily be evaluated both qualitatively by methylene blue staining scores, and quantitatively by chronic assay. Visually observed corneal epithelial thinning was confirmed by scanning electron microscopy (SEM) to be due to loss of epithelial integrity.Using a 3 % chondroitin sulfate solution, an already proven effective agent for dry eye, this model effectively demonstrated an 80 % inhibition in the development of methylene blue positive lesion after a period of only 2 hours. This short term dry eye model is valuable in primarily screening the efficacy of potential therapeutic agents in the prevention and treatment of dry eye.