Bactericidal activity of the alternative complement pathway generated from 11 isolated plasma proteins.
Bactericidal activity of the alternative complement pathway generated from 11 isolated plasma proteins.
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DOI：
10.1084/jem.149.4.870
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发表时间：
1979-04-01
期刊：
影响因子：
--
通讯作者：
Müller-Eberhard HJ
中科院分区：
文献类型：
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作者：
Schreiber RD;Morrison DC;Podack ER;Müller-Eberhard HJ
将大肠杆菌 K12 W1485 暴露于由 11 种分离途径蛋白组装而成的细胞溶解替代途径中，导致细菌被杀死，其活力丧失就证明了这一点。细菌的裂解需要将溶菌酶引入反应混合物中。分离系统中溶菌的时程和剂量依赖性与 C4 耗尽血清中的相同。该途径的溶菌活性高度依赖于该途径蛋白的浓度，并且在1:16的生理浓度下变得微不足道。杀死和裂解的细菌的电子显微镜观察显示许多补体膜损伤和外磷脂膜的部分崩解。扫描电镜显示，被杀死的细菌体积增大，部分塌陷，并呈现出不规则的表面突起。裂解的细菌破碎并呈现多态性。这项研究表明，在没有免疫球蛋白的情况下，替代途径有可能根除革兰氏阴性菌。
Exposure of E. coli K12 W1485 to the cytolytic alternative pathway assembled from the 11 isolated pathway proteins resulted in killing of the bacteria, as evidenced by loss of viability. Lysis of the bacteria required introduction of lysozyme into the reaction mixture. The time- course and dose dependency of bacteriolysis in the isolated system were identical to those in C4-depleted serum. The bacteriolytic activity of the pathway was highly dependent on the concentration of the pathway proteins and became insignificant at 1:16 physiological concentration. Electron microscopic visualization of killed and of lysed bacteria revealed numerous complement membrane lesions and partial disintegration of the outer phospholipid membrane. Scanning electron microscopy showed that killed bacteria were enlarged, partially collapsed and exhibited irregular surface protrusions. Lysed bacteria were fragmented and appeared polymorphic. This study demonstrates that the alternative pathway, in absence of immunoglobulins, has the potential or eradicating gram-negative bacteria.