Brain gene expression changes elicited by peripheral vitellogenin knockdown in the honey bee.
Brain gene expression changes elicited by peripheral vitellogenin knockdown in the honey bee.
复制标题
蜜蜂外周卵黄原蛋白敲低引起的大脑基因表达变化。
DOI：
10.1111/imb.12043
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发表时间：
2013
影响因子：
2.6
通讯作者：
Robinson,GE
中科院分区：
文献类型：
--
作者：
Wheeler,MM;Ament,SA;Rodriguez-Zas,SL;Robinson,GE
卵黄原蛋白（Vg）是一种众所周知的卵黄蛋白前体。Vg也有调节成年蜜蜂工蜂行为成熟的功能，但其发挥这种新作用的潜在分子机制在很大程度上是未知的。我们使用RNA干扰（RNAi）和脑转录组分析来降低腹部卵黄蛋白原（vg），以深入了解vg如何影响蜜蜂的行为成熟。我们发现，vgknockdown导致蜜蜂大脑中广泛的基因表达变化，其中大部分转录反应涉及中枢生物功能（如能量代谢）的变化。Vgknockdown针对的是许多相同的基因，这些基因表现出自然的、成熟相关的差异，但这两个对比中基因的变化方向并不相关。相比之下，vgknockdown针对的是许多受少年激素（JH）调控的相同基因，并且在这两个对比中，基因的变化方向具有显著的相关性。这些结果表明，JH和Vg在蜜蜂行为成熟的调控中存在紧密的协同调节关系，并在基因组水平上表现出来，表明这两个生理因素通过共同的途径调节大脑基因的表达和行为。
Vitellogenin (Vg) is best known as a yolk protein precursor. Vg also functions to regulate behavioural maturation in adult honey bee workers, but the underlying molecular mechanisms by which it exerts this novel effect are largely unknown. We used abdominalvitellogenin(vg) knockdown with RNA interference (RNAi) and brain transcriptomic profiling to gain insights into how Vg influences honey bee behavioural maturation. We found thatvgknockdown caused extensive gene expression changes in the bee brain, with much of this transcriptional response involving changes in central biological functions such as energy metabolism.vgknockdown targeted many of the same genes that show natural, maturation‐related differences, but the direction of change for the genes in these two contrasts was not correlated. By contrast,vgknockdown targeted many of the same genes that are regulated by juvenile hormone (JH) and there was a significant correlation for the direction of change for the genes in these two contrasts. These results indicate that the tight coregulatory relationship that exists between JH and Vg in the regulation of honey bee behavioural maturation is manifest at the genomic level and suggest that these two physiological factors act through common pathways to regulate brain gene expression and behaviour.