Transgenic alfalfa plants expressing AtNDPK2 exhibit increased growth and tolerance to abiotic stresses.
Transgenic alfalfa plants expressing AtNDPK2 exhibit increased growth and tolerance to abiotic stresses.
复制标题
DOI：
10.1016/j.plaphy.2014.08.025
复制
发表时间：
2014-11
期刊：
影响因子：
--
通讯作者：
Zhi Wang;Hongbing Li;Qingbo Ke;J. Jeong;Haeng-Soon Lee;Bingcheng Xu;Xi-ping Deng;Y. Lim;S. Kwak
中科院分区：
文献类型：
--
作者：
Zhi Wang;Hongbing Li;Qingbo Ke;J. Jeong;Haeng-Soon Lee;Bingcheng Xu;Xi-ping Deng;Y. Lim;S. Kwak
In this study, we generated and evaluated transgenic alfalfa plants (MedicagosativaL. cv. Xinjiang Daye) expressing the Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) gene under the control of the oxidative stress-inducibleSWPA2promoter (referred to as SN plants) to develop plants with enhanced tolerance to various abiotic stresses. We selected two SN plants (SN4 and SN7) according to the expression levels ofAtNDPK2and the enzyme activity of NDPK in response to methyl viologen (MV)-mediated oxidative stress treatment using leaf discs for further characterization. SN plants showed enhanced tolerance to high temperature, NaCl, and drought stress on the whole-plant level. When the plants were subjected to high temperature treatment (42 °C for 24 h), the non-transgenic (NT) plants were severely wilted, whereas the SN plants were not affected because they maintained high relative water and chlorophyll contents. The SN plants also showed significantly higher tolerance to 250 mM NaCl and water stress treatment than the NT plants. In addition, the SN plants exhibited better plant growth through increased expression of auxin-related indole acetic acid (IAA) genes (MsIAA3,MsIAA5,MsIAA6,MsIAA7, andMsIAA16) under normal growth conditions compared to NT plants. The results suggest that induced overexpression ofAtNDPK2in alfalfa will be useful for increasing biomass production under various abiotic stress conditions.
