A Dynamic Defect Generation Strategy for Efficient Enzyme Immobilization in Robust Metal-Organic Framework for Catalytic Hydrolysis and Chiral Resolution.
A Dynamic Defect Generation Strategy for Efficient Enzyme Immobilization in Robust Metal-Organic Framework for Catalytic Hydrolysis and Chiral Resolution.
复制标题
用于催化水解和手性拆分的稳健金属有机框架中有效酶固定的动态缺陷生成策略。
DOI：
10.1002/anie.202302436
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发表时间：
2023
影响因子：
--
通讯作者：
Yao Chen
中科院分区：
文献类型：
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作者：
Yifan Feng;Ruixuan Shi;Mingfang Yang;Yunlong Zheng;Zhenjie Zhang;Yao Chen
酶固定化已被证明是促进生物活性分子产业化的有利方案，但仍面临着巨大的挑战。为了应对这一挑战，我们利用酶介导的金属-有机骨架(MOF)的解离平衡，创建了一种用于酶固定化的动态缺陷生成策略。酶可以作为大分子配体，与原始配体竞争配位，同时释放金属基团簇产生缺陷，从而促进酶从表面向内部的逐渐运输。各种酶可以有效地固定在MOF中，从而提供具有良好的酶活性、保护性能和优异的可重用性的复合材料。此外，还可以产生能够进行高效级联反应的多酶生物反应器。这项研究为构建包含不同类型酶的高效生物催化剂提供了新的机会。
Enzyme immobilization has been demonstrated to be a favorable protocol for promoting the industrialization of bioactive molecules, but still with formidable challenge. Addressing this challenge, we create a dynamic defect generation strategy for enzyme immobilization by using the dissociation equilibrium of metal-organic frameworks (MOFs) mediated by enzymes. Enzymes can act as "macro ligands" to generate competitive coordination against original ligands, along with the release of metal clusters of MOFs to generate defects, hence promoting the gradual transport of enzymes from the surface to inside. Various enzymes can be efficiently immobilized in MOFs to afford composites with good enzymatic activities, protective performances and exceptional reusabilities. Moreover, multienzyme bioreactors capable of efficient cascade reactions can also be generated. This study provides new opportunities to construct highly efficient biocatalysts incorporating different types of enzymes.