Inhibition of induced melanogenesis in Cloudman melanoma cells by four phenotypic modifiers.
Inhibition of induced melanogenesis in Cloudman melanoma cells by four phenotypic modifiers.
复制标题
四种表型修饰剂抑制 Cloudman 黑色素瘤细胞中诱导的黑色素生成。
DOI：
10.1016/0014-4827(90)90007-w
复制
发表时间：
1990
影响因子：
3.7
通讯作者：
Pawelek,JM
中科院分区：
文献类型：
--
作者：
Orlow,SJ;Chakraborty,AK;Boissy,RE;Pawelek,JM
Retinoic acid, hexamethylene bisacetamide, sodium butyrate, and dimethylsulfoxide, four compounds which modulate phenotypic expression in a variety of neoplastic cell lines, all inhibited the induction of tyrosinase activity and melanogenesis by the combination of melanocyte-stimulating hormone and isobutylmethylxanthine in Cloudman S91 melanoma cells. Results were the same in assays of whole cells or in extracts made from them. Only retinoic acid, however, was effective at inhibiting the activation of dopachrome isomerase, another regulatory enzyme in melanogenesis. Despite inhibiting the effects of melanocyte-stimulating hormone (MSH) and isobutylmethylxanthine on tyrosinase activity, all of the agents tested increased the binding of MSH to intact cells. Ultrastructural analysis of treated cells following DOPA cytochemistry revealed that both retinoic acid and hexamethylene bisacetamide arrested melanosomal maturation at stage I–II. Retinoic acid resulted in a derangement of melanosomal structure. The specificity of these agents for preventing the induction of melanogenesis makes them powerful tools for the dissection of this complex cellular process.
