Overexpression of an alternative oxidase gene, OsAOX1a, improves cold tolerance in Oryza sativa L.
Overexpression of an alternative oxidase gene, OsAOX1a, improves cold tolerance in Oryza sativa L.
复制标题
替代氧化酶基因 OsAOX1a 的过度表达可提高水稻的耐寒性。
DOI：
10.4238/2013.november.11.4
复制
发表时间：
2013-01-01
影响因子：
0.4
通讯作者：
Yang, J. B.
中科院分区：
文献类型：
--
作者：
Li, C. R.;Liang, D. D.;Yang, J. B.
低温是水稻栽培和生产中的主要环境胁迫。交替氧化酶1（AOX1）基因在提高冷适应性的基因工程中具有潜在的重要意义。然而，以前的研究主要集中在双子叶植物拟南芥和烟草，而水稻的功能研究是有限的。本研究克隆了一个水稻低温反应型AOX1基因OsAOX1a。获得了OsAOX1a过表达的转基因水稻植株。我们发现OsAOX1a过表达可以强烈地增强幼苗的冷生长，特别是在根延伸方面。然而，在正常条件下，转基因植物和对照植物之间的生长没有差异。此外，还测定了转基因植株在低温处理后的膜脂过氧化和离子渗漏率。OsAOX1a的过表达降低了这两个因子，表明OsAOX1a可以降低低温胁迫下的氧化损伤。以上结果表明，过量表达OsAOX1a能够提高水稻在低温胁迫下的生长表现，其机制可能与减少活性氧的产生和氧化损伤有关。
Low temperature is a major environmental stress in rice cultivating and production. The alternative oxidase 1 (AOX1) gene is potentially important for genetic engineering to increase cold adaptation. However, previous studies related to this effect have mostly focused on the dicot plants Arabidopsis and tobacco, whereas functional research on rice is limited. In this study, we cloned a rice predominant cold-response AOX1 gene, OsAOX1a. Transgenic rice plants with overexpression of OsAOX1a were obtained. We found that OsAOX1a overexpression could strongly enhance the cold growth of seedlings, especially with respect to root extension. However, growth between transgenic and control plants did not differ under normal conditions. Furthermore, the lipid peroxidation and ion leakage rate were determined after cold treatment in transgenic plants. Both factors were reduced by OsAOX1a overexpression, which revealed that OsAOX1a could reduce oxidative damage under cold stress. Taken together, our results suggested that overexpressing OsAOX1a could improve growth performance of rice under cold stress, which might be closely related to the reduction of reactive oxygen species generation and oxidative damage.