Detection of a male-specific sequence in nonhuman primates through use of the polymerase chain reaction.
Detection of a male-specific sequence in nonhuman primates through use of the polymerase chain reaction.
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通过使用聚合酶链式反应检测非人灵长类动物中的雄性特异性序列。
DOI：
10.1159/000133579
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Pallavicini,MG
中科院分区：
文献类型：
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作者：
Reitsma,MJ;Harrison,MR;Pallavicini,MG
性别特异性DNA序列可用于检测和监测移植受者的嵌合现象，这些受者接受了性别不匹配的供体细胞。非人类灵长类动物经常被用作实验移植模型，因为它们在进化上接近人类，并且它们的身体特征与人类相似。不幸的是，目前还没有基于dna的分子检测策略来监测猴子和狒狒性别不匹配移植的植入情况。我们描述了一种基于聚合酶链反应的检测方法的发展，以检测恒河猴（Macaca mulatta）和橄榄狒狒（Papio cynocephalus）中存在的174 bp雄性特异性序列。该试验足够灵敏，可以在104个女性细胞的背景下检测到10个男性细胞。在描述的分析条件下，人类序列不扩增。扩增的DNA序列与人类基因组中睾丸决定因子位点附近的序列同源度为82%，表明该区域具有高度的进化保守性。
Sex-specific DNA sequences are useful for detecting and monitoring chimerism in transplant recipients that had received sex-mismatched donor cells. Nonhuman primates are often used as experimental transplant models because of their evolutionary proximity, and the similarity of their physical characteristics, to those of humans. Unfortunately, DNA-based molecular detection strategies to monitor engraftment in sex-mismatched transplants in monkeys and baboons have not been available. We describe development of a polymerase chain reaction-based assay to detect a 174-bp male-specific sequence present in the rhesus monkey (Macaca mulatta) and olive baboon (Papio cynocephalus). The assay is sufficiently sensitive to allow detection of 10 male cells against a background of 104female cells. Human sequence is not amplified under the described assay conditions. The amplified DNA sequence is 82% homologous to a sequence located near the testis-determining factor locus in the human genome, suggesting a high degree of evolutionary conservation in this region.