Differentiation of female Oct4‐GFP embryonic stem cells into germ lineage cells
Differentiation of female Oct4‐GFP embryonic stem cells into germ lineage cells
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DOI：
10.1002/cbin.10918
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发表时间：
2018-04
影响因子：
3.9
通讯作者：
Xin Ma;Peng Li;Xiang Sun;Yifeng Sun;Rong Hu;P. Yuan
中科院分区：
文献类型：
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作者：
Xin Ma;Peng Li;Xiang Sun;Yifeng Sun;Rong Hu;P. Yuan
由于目前哺乳动物的不育率很高，因此有必要探索提高哺乳动物繁殖力，特别是雌性繁殖力的有效途径。使用雌性Oct 4-GFP胚胎干细胞，我们模拟体内发育过程，将ES细胞诱导成上胚层细胞样细胞（EpiLC），然后诱导成原始生殖细胞样细胞（PGCLC）。高效地获得了显示典型PGC标记和表观遗传修饰的GFP阳性PGCLC。进一步将GFP阳性PGCLC与天然卵巢细胞混合移植到不育小鼠卵巢中，发现MVH和GFP阳性细胞均可在卵巢中发育，但未观察到发育后期的生殖细胞。这项研究表明，Oct 4-GFP ES细胞可能仅适用于追踪早期生殖细胞发育。
Due to high infertility ratio nowadays, it is essential to explore efficient ways of enhancing mammalian reproductivity, in particular female reproductivity. Using female Oct4‐GFP embryonic stem cells, we mimic the in vivo development procedure to induce ES cells into epiblast cell‐like cells (EpiLCs) and then primordial germ cell‐like cells (PGCLCs). GFP positive PGCLCs that showed typical PGC markers and epigenetic modification were efficiently obtained. Further transplantation of the GFP positive PGCLC and native ovary cell mixture into ovary of infertile mice revealed that both MVH and GFP positive cells could be developed in ovary, but no later developmental stage germ cells were observed. This study suggested that Oct4‐GFP ES cells may be only suitable for tracing early germ cell development.