Comparison of the performance characteristics of poly(dimethylsiloxane) and Pyrex microchip electrophoresis devices for peptide separations
Comparison of the performance characteristics of poly(dimethylsiloxane) and Pyrex microchip electrophoresis devices for peptide separations
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DOI：
10.1016/s0021-9673(03)00722-2
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发表时间：
2003-07-04
影响因子：
4.1
通讯作者：
Lunte, SM
中科院分区：
文献类型：
--
作者：
Lacher, NA;de Rooij, NF;Lunte, SM
对聚二甲基硅氧烷（PDMS）和Pyrex微芯片上荧光标记肽和氨基酸的电泳分离进行了比较研究。对每个微芯片基底的分离参数进行了比较，包括电渗流、板数、分辨率和检测限。缓冲液组成对分离的影响也进行了研究。对于大多数肽与两种底物都获得了可接受的分离;然而，PDMS芯片表现出低得多的分离效率和较长的分析时间。(C)2003 Elsevier B. V.保留所有权利。
A comparative study of electrophoretic separations of fluorescently labeled peptides and amino acids on poly(dimethylsiloxane) (PDMS) and Pyrex microchips is presented. The separation parameters for each microchip substrate were compared, including electroosmotic flow, plate numbers, resolution, and limits of detection. The effect of buffer composition on the separation was also investigated. Acceptable separations were obtained for most peptides with both substrates; however, PDMS chips exhibited much lower separation efficiencies and longer analysis times. (C) 2003 Elsevier B.V. All rights reserved.