Immunohistochemical analysis of pro-apoptotic Bid level in chronic hepatitis, hepatocellular carcinoma and liver metastases
Immunohistochemical analysis of pro-apoptotic Bid level in chronic hepatitis, hepatocellular carcinoma and liver metastases
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DOI：
10.1016/s0304-3835(01)00630-9
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发表时间：
2001-10-22
期刊：
影响因子：
9.7
通讯作者：
Lau, WY
中科院分区：
文献类型：
--
作者：
Chen, GG;Lai, PBS;Lau, WY
Bid是Bcl-2家族的成员，通过诱导促凋亡因子的释放介导凋亡。Bid在肝脏疾病中的表达尚未研究。本研究评估了各种肝病中的Bid水平，包括肝细胞癌（HCC）、结直肠癌肝转移、慢性肝炎和肝硬化。Bid在同一患者癌组织中的表达低于相应的非癌组织。在低分化肿瘤患者的一些局部肿瘤肝组织中发现Bid抗体的强染色。在慢性肝炎和肝硬化患者中，存在梯度肿瘤发展中心，从中心的中间到边缘，与Bid抗体的反应呈梯度增加。肝癌非肿瘤组织中也存在梯度肿瘤发展中心，提示慢性肝炎中出现梯度肿瘤发展中心可能是肝癌发展的早期病理标志。Bid在肝转移瘤组织的上皮细胞中也有表达，其表达通常强于非肿瘤性肝组织。在这些转移性细胞中，Bid的重核染色并不少见。原发性和继发性肝肿瘤之间的不同染色模式可能反映了肿瘤来源和细胞类型的差异。转移性细胞的细胞核，虽然Bid阳性，但仍显示出相当大的有丝分裂活性，表明它们处于活跃的增殖中，而不是在被认为是凋亡的途径上。总之，这项研究表明，Bid水平降低，在肝癌，除了在低分化的肝癌细胞可能会经历一个过程中的凋亡或坏死。在慢性肝炎、肝硬化和肝癌的非肿瘤组织中存在梯度肿瘤发展中心，可能是细胞表型发生癌变的病理标志。(C)2001爱思唯尔科学爱尔兰有限公司保留所有权利。
Bid, a member of the Bcl-2 family, mediates apoptosis by inducing the release of proapoptotic factors. The expression of Bid in liver diseases has not been investigated. This study evaluated Bid level in various liver diseases including hepatocellular carcinoma (HCC), liver metastases from colorectal cancer, chronic hepatitis and liver cirrhosis. The expression of Bid in tumorous tissues of HCC was lower than that in their corresponding non-tumorous tissues from the same patient. Heavy staining with Bid antibody was found in some localized tumorous liver tissues from patients with poorly differentiated tumors. In patients with chronic hepatitis and liver cirrhosis, there were gradient tumor-development centers, a gradient increase in reaction with Bid antibody from the middle of the center to its edge. The gradient tumor-development center was also found in non-tumorous tissues of HCC, suggesting that occurrence of this center in chronic hepatitis might be an early pathologic sign of HCC development. Bid was also expressed in the epithelial cells in tissues from liver metastases and their expression was often stronger than in the non-tumorous liver tissues. Heavy nuclear staining of Bid was not uncommon in these metastatic cells. The different patterns of staining between primary and secondary liver tumors may reflect a difference in tumor origin and in cell type. Nuclei of metastatic cells, though positive for Bid, still showed a considerable mitotic activity, indicating that they were in active proliferation rather than on a pathway deemed to be apoptotic. In conclusion, this study shows that the Bid level is decreased in HCC except in poorly differentiated HCC in which cells may undergo a process of apoptosis or necrosis. The existence of gradient tumor-development center in chronic hepatitis, liver cirrhosis and nontumorous tissues from HCC may serve as a pathologic marker of a carcinogenic change of cell phenotypes. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.