LOCALIZATION OF [I-125] OMEGA-CONOTOXIN GVIA BINDING IN HUMAN HIPPOCAMPUS AND CEREBELLUM
LOCALIZATION OF [I-125] OMEGA-CONOTOXIN GVIA BINDING IN HUMAN HIPPOCAMPUS AND CEREBELLUM
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DOI：
10.1097/00001756-199309150-00011
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发表时间：
1993-09-30
期刊：
影响因子：
1.7
通讯作者：
FILLOUS, F
中科院分区：
文献类型：
--
作者：
ALBENSI, BC;RYUJIN, KT;FILLOUS, F
多肽毒素omega-conooxGVIA(omega-CgTx)是脑内N型钙通道的高亲和力配体。我们已经使用[I-125]omega-CgTx通过放射自显影来定位人类海马体和小脑中的N型通道。在不同条件下，用[I-125]omega-CgTx标记10微米厚的人小脑和海马区组织切片。与人小脑的特异性结合几乎是不可逆的和饱和的。它能被N通道拮抗剂omega-conooxin MVIIA取代，但不能被L或P通道配体取代。结合部位分布不均，分子层结合密度大于小脑颗粒细胞层，在海马区有明显的层状结构。[I-125]omega-CgTx有望成为研究人脑组织N型钙通道的有用工具。
THE peptide toxin omega-conotoxin GVIA (omega-CgTx) has been shown to be a high affinity ligand for N-type calcium channels in the brain. We have employed [I-125]omega-CgTx to localize N-type channels in human hippocampus and cerebellum using autoradiography. Ten micron thick slide-mounted tissue sections of human cerebellum and hippocampus were labeled with [I-125]omega-CgTx under various conditions. Specific binding to human cerebellum was virtually irreversible and saturable. It was displaceable by the N-channel antagonist, omega-conotoxin MVIIA, but not by L- or P- channel ligands. Binding sites were heterogeneously distributed with denser binding in the molecular layer than the granule cell layer of cerebellum and with specific laminar patterns evident in the hippocampus. [I-125]omega-CgTx should be a useful tool for the study Of N-type calcium channels in human brain tissue.