Detection of fractalkine in human seminal plasma and its role in infertile patients.
Detection of fractalkine in human seminal plasma and its role in infertile patients.
复制标题
人精浆中 fractalkine 的检测及其在不孕患者中的作用。
作者：
Qing Zhang;K. Shimoya;Y. Ohta;R. Chin;Kumiko Tenma;Shigeyuki Isaka;H. Nakamura;M. Koyama;C. Azuma;Y. Murata
背景
Fractalkine是一种较新发现的CX(3)C趋化因子，对T细胞、单核细胞和自然杀伤细胞具有趋化作用。一些报告已经证明精浆中趋化因子水平与精液质量之间存在关联。检测了正常精液和伴有或不伴有弱精子症的男性不育患者精液中的Fractalkine水平，并与精子活力和形态进行了相关分析。
方法和结果
Western印迹分析显示，精浆中存在Fractalkine蛋白。弱精子症不育患者精浆Fractalkine滴度(0.64±0.04微克/毫升；n=58)低于无弱精子症患者(0.94±0.10微克/毫升；P<0.01)和正常生育男性(1.04±0.07微克/毫升；n=10，P<0.001)。患有和不患有白精症的患者的Fractalkine水平没有显著差异。精浆中Fractalkine水平与IL-8水平无明显相关性。精子活力与Fractalkine浓度呈正相关(R(2)=0.14P&0.001)。用Cx(3)CR特异性引物证实精液中存在Cx(3)CR阳性的白细胞。
结论
这些结果表明，Fractalkine是一种与精子活力和Cx(3)CR阳性白细胞向精液迁移有关的趋化因子。
BACKGROUND
Fractalkine is a relatively newly discovered CX(3)C chemokine, which is a chemoattractant for T cells, monocytes and natural killer cells. Several reports have demonstrated the association between chemokine levels in seminal plasma and semen quality. The fractalkine levels in ejaculates from normal donors and infertile male patients with or without asthenozoospermia, were examined and correlated with sperm motility and morphology.
METHODS AND RESULTS
Western blot analysis showed fractalkine protein to be present in the seminal plasma. Fractalkine titres in the seminal plasma of infertile men with asthenozoospermia (0.64 +/- 0.04 microg/ml; n = 58) were lower than those in patients without asthenozoospermia (0.94 +/- 0.10 microg/ml; n = 22, P < 0.01) and fertile donors (1.04 +/- 0.07 microg/ml; n = 10, P < 0.001). There was no significant difference between fractalkine levels in patients with and without leukospermia. No significant correlation was found between fractalkine and interleukin-8 levels in seminal plasma. Sperm motility was positively correlated (R(2) = 0.14, P < 0.001) with fractalkine concentration. The existence of CX(3)CR-positive leukocytes in semen was confirmed using specific primers for CX(3)CR.
CONCLUSIONS
These results suggest that fractalkine is a chemokine associated with sperm motility and the migration of CX(3)CR-positive leukocytes into semen.
精子穿透测定中反映的脓精症和精液细菌学与精子功能的关系。
影响因子：
6.7
作者：
Berger,RE;Karp,LE;Williamson,RA;Koehler,J;Moore,DE;Holmes,KK
通讯作者：
Holmes,KK
粒细胞弹性蛋白酶作为白细胞精子症精浆标记物的评估。
影响因子：
6.7
作者：
Wolff,H;Anderson,DJ
通讯作者：
Anderson,DJ
白细胞精子症与精液质量差有关。
影响因子：
6.7
作者：
Wolff,H;Politch,JA;Martinez,A;Haimovici,F;Hill,JA;Anderson,DJ
通讯作者：
Anderson,DJ