High-Throughput DNA Microarray Detection of Pathogenic Bacteria in Shallow Well Groundwater in the Kathmandu Valley, Nepal
High-Throughput DNA Microarray Detection of Pathogenic Bacteria in Shallow Well Groundwater in the Kathmandu Valley, Nepal
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DOI：
10.1007/s00284-014-0681-x
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发表时间：
2014-08
影响因子：
2.6
通讯作者：
D. Inoue;Takuji Hinoura;N. Suzuki;Junqin Pang;R. Malla;Sadhana Shrestha;S. Chapagain;Hiroaki Matsuzawa;Takashi Nakamura;Yasuhiro Tanaka;M. Ike;K. Nishida;K. Sei
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文献类型：
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作者：
D. Inoue;Takuji Hinoura;N. Suzuki;Junqin Pang;R. Malla;Sadhana Shrestha;S. Chapagain;Hiroaki Matsuzawa;Takashi Nakamura;Yasuhiro Tanaka;M. Ike;K. Nishida;K. Sei
在尼泊尔的加德满都谷地，由于严重依赖地下水作为饮用水和其他生活用水，地下水的微生物污染是一个严重的问题。本研究应用基因芯片技术对加德满都谷地浅井地下水中941个致病菌种/群进行了全面调查。水质测量发现，在11个调查的地下水样本中，有10个样本受到大肠菌（粪便）污染，一些样本受到氮污染。DNA微阵列分析结果显示，11个地下水样品中有9个样品含有1-37个病原菌种/群，其中生物安全二级病原菌1-27个。虽然检测到的病原体包括几种粪便和动物相关的病原体，但属于军团菌和节杆菌属的病原体被认为与粪便没有直接关系，但在早期检测到。这项研究可以提供一个整体的致病性细菌污染在加德满都山谷的粗略图片，并证明了DNA微阵列分析作为一个全面的筛选工具的各种致病性细菌的有用性。
Because of heavy dependence on groundwater for drinking water and other domestic use, microbial contamination of groundwater is a serious problem in the Kathmandu Valley, Nepal. This study investigated comprehensively the occurrence of pathogenic bacteria in shallow well groundwater in the Kathmandu Valley by applying DNA microarray analysis targeting 941 pathogenic bacterial species/groups. Water quality measurements found significant coliform (fecal) contamination in 10 of the 11 investigated groundwater samples and significant nitrogen contamination in some samples. The results of DNA microarray analysis revealed the presence of 1–37 pathogen species/groups, including 1–27 biosafety level 2 ones, in 9 of the 11 groundwater samples. While the detected pathogens included several feces- and animal-related ones, those belonging toLegionellaandArthrobacter, which were considered not to be directly associated with feces, were detected prevalently. This study could provide a rough picture of overall pathogenic bacterial contamination in the Kathmandu Valley, and demonstrated the usefulness of DNA microarray analysis as a comprehensive screening tool of a wide variety of pathogenic bacteria.