Structural and biochemical changes in dermis of sea cucumber (Stichopus japonicus) during autolysis in response to cutting the body wall
Structural and biochemical changes in dermis of sea cucumber (Stichopus japonicus) during autolysis in response to cutting the body wall
复制标题
海参（Stichopus japonicus）真皮在自溶过程中响应切割体壁的结构和生化变化
DOI：
10.1016/j.foodchem.2017.08.071
复制
发表时间：
2018-02-01
期刊：
影响因子：
8.8
通讯作者：
Shahidi, Fereidoon
中科院分区：
文献类型：
--
作者：
Liu, Yu-Xin;Zhou, Da-Yong;Shahidi, Fereidoon
海参体壁的自溶是由其结构成分的内源性蛋白质水解引起的。但海参体壁主要结构成分胶原原纤维、胶原纤维和微纤维在自溶过程中的变化尚不清楚。海参（S.采用电子显微镜、差示扫描量热法、红外光谱、电泳和化学分析等方法研究了其真皮组织的结构和生化变化。在自溶过程中，胶原纤维和微纤维逐渐降解。相比之下，对微纤维的损伤更明显。在大量自溶时，胶原纤维由于纤维间桥断裂而解聚成胶原纤维束和单个纤维。同时，胶原纤维过度展开。然而，胶原蛋白单体仅有轻微损伤。因此，胶原纤维、胶原原纤维和微纤维的结构损伤而不是单体胶原导致了S的自溶。真皮
The autolysis of sea cucumber body wall is caused by endogenous proteolysis of its structural elements. However, changes in collagen fibrils, collagen fibres and microfibrils, the major structural elements in sea cucumber body wall during autolysis are less clear. Autolysis of sea cucumber (S. japonicus) was induced by cutting the body wall, and the structural and biochemical changes in its dermis were investigated using electron microscopy, differential scanning calorimetry, infrared spectroscopy, electrophoresis, and chemical analysis. During autolysis, both collagen fibres and microfibrils gradually degraded. In contrast, damage to microfibrils was more pronounced. Upon massive autolysis, collagen fibres disaggregated into collagen fibril bundles and individual fibrils due to the fracture of interfibrillar bridges. Meanwhile, excessive unfolding of collagen fibrils occurred. However, there was only slight damage to collagen monomers. Therefore, structural damage in collagen fibres, collagen fibrils and microfibrils rather than monomeric collagen accounts for autolysis of S. japonicus dermis.