In Vitro Morphogenesis and Differentiation of Human Intestinal Epithelium in a Gut-on-a-Chip.
In Vitro Morphogenesis and Differentiation of Human Intestinal Epithelium in a Gut-on-a-Chip.
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芯片肠道中人肠上皮的体外形态发生和分化。
DOI：
10.1007/978-1-0716-3076-1_15
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Kim,HyunJung
中科院分区：
文献类型：
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作者：
Shin,Woojung;Kim,HyunJung
体外三维上皮结构和细胞分化的建立对于再现人体肠道的体内相关结构和功能是必要的。在这里，我们描述了一个实验方案，以建立一个器官模拟肠芯片上的微器件，允许使用Caco-2细胞或肠类器官细胞诱导人肠上皮的3D形态发生。在生理流动和物理运动下，肠上皮在芯片上肠中自发地重建3D上皮形态，其提供增强的粘液产生、上皮屏障和纵向宿主-微生物共培养。该方案可以提供可实施的策略，以推进传统的体外静态培养、人类微生物组研究和药理学测试。
The establishment of a three-dimensional (3D) epithelial structure and cytodifferentiation in vitro is necessary to recapitulate in vivo-relevant structure and function of the human intestine. Here, we describe an experimental protocol to build an organomimetic gut-on-a-chip microdevice that allows inducing 3D morphogenesis of human intestinal epithelium using Caco-2 cells or intestinal organoid cells. Under physiological flow and physical motions, intestinal epithelium spontaneously recreates 3D epithelial morphology in a gut-on-a-chip that offers enhanced mucus production, epithelial barrier, and longitudinal host-microbe co-culture. This protocol may provide implementable strategies to advance traditional in vitro static cultures, human microbiome studies, and pharmacological testing.