Arterial baroreflex dysfunction promotes atherosclerosis in rats
Arterial baroreflex dysfunction promotes atherosclerosis in rats
复制标题
动脉压力反射功能障碍促进大鼠动脉粥样硬化。
DOI：
10.1016/j.atherosclerosis.2005.03.037
复制
发表时间：
2005-11-01
期刊：
影响因子：
5.3
通讯作者：
Su, DF
中科院分区：
文献类型：
--
作者：
Cai, GJ;Miao, CY;Su, DF
本研究旨在证实动脉压力反射功能障碍促进动脉粥样硬化发展的假设。实验1：用计算机血压监测系统测量30只清醒状态下的SD大鼠的压力反射敏感性(BRS)。 4周后，给大鼠注射维生素D-3，并喂食8周的高胆固醇饮食以诱导动脉粥样硬化。取出心脏和主动脉进行病理检查。 SD大鼠的BRS与冠状动脉粥样硬化评分（r=-0.464，P < 0.0 1）或主动脉粥样硬化评分（r= -0.524，P < 0.01）呈负相关。实验2：对SD大鼠进行去主动脉神经支配(SAD)。然后还诱发动脉粥样硬化。 SAD大鼠动脉粥样硬化评分显着高于假手术大鼠（主动脉评分：1.50±0.41 vs 1. 10±0.39，P < 0.05；冠状动脉评分：1.70±0.35 vs 1.25±0.54，P < 0.05）。采用免疫组化和Western blotting方法发现，与假手术大鼠相比，SAD大鼠冠状动脉和主动脉中C反应蛋白、细胞间粘附分子-1和血管细胞粘附分子-1的表达增加。这些结果表明，动脉压力感受反射功能障碍促进了大鼠动脉粥样硬化的发展，并且炎症可能参与了这一过程。 (c) 2005 Elsevier Ireland Ltd. 保留所有权利。
The present study was designed to testify the hypothesis that arterial baroreflex dysfunction promotes the development of atherosclerosis. Experiment 1: the baroreflex sensitivity (BRS) was measured in 30 Sprague-Dawley (SD) rats in conscious state with a computerized blood pressure monitoring system. Four weeks later, the rats were administered with Vitamin D-3, and fed with the high-cholesterol diet for 8 weeks to induce atherosclerosis. The hearts and aortae were removed for pathological examination. A negative correlation was found between BRS and the scores of coronary (r= -0.464, P < 0.0 1) or aortic atherosclerosis (r= -0.524, P < 0.01) in SD rats. Experiment 2: sinoaortic denervation (SAD) was performed in SD rats. Then atherosclerosis was also induced. The atherosclerosis scores in SAD rats were significantly higher than those in sham-operated rats (aortic score: 1.50 +/- 0.41 versus 1. 10 +/- 0.39, P < 0.05; coronary score: 1.70 0.35 versus 1.25 +/- 0.54, P < 0.05). Using immunohistochemistry and Western blotting methods, it was found that the expressions of C-reactive protein, intercellular adhesion molecule-1 and vascular-cell adhesion molecule-1 in coronary artery and aorta were increased in SAD rats compared with sham-operated rats. These results indicate that arterial baroreflex dysfunction promotes the development of atherosclerosis in rats, and that inflammation may be involved in this process. (c) 2005 Elsevier Ireland Ltd. All rights reserved.