Peptidoglycan from Akkermansia muciniphila MucT: chemical structure and immunostimulatory properties of muropeptides.
Peptidoglycan from Akkermansia muciniphila MucT: chemical structure and immunostimulatory properties of muropeptides.
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来自 Akkermansia muciniphila MucT 的肽聚糖：muropeptides 的化学结构和免疫刺激特性。
DOI：
10.1093/glycob/cwac027
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发表时间：
2022
期刊：
影响因子：
4.3
通讯作者：
Garcia-Vello P
中科院分区：
文献类型：
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作者：
Garcia-Vello P
Akkermansia muciniphilais 是一种肠道共生体，已知可以改善小鼠和人类的肠道屏障功能。多种细胞包膜成分已被鉴定在 A 的免疫信号传导中发挥关键作用。 muciniphila，但肽聚糖（PG）的化学成分和作用仍然难以捉摸。在这里，我们从A中分离出PG片段。 muciniphilaMucT(ATCC BAA-835),分析其组成并评估其免疫信号传导能力。从结构上看，A的PG。 muciniphilawas 因存在一些非乙酰化葡萄糖胺残基而值得注意，这可能源于 N-乙酰葡萄糖胺的脱乙酰化。一些N-乙酰胞壁酸(MurNAc)亚基被O-乙酰化。免疫学测定表明，A 释放出胞肽。 muciniphilaPG 均可激活细胞内 NOD1 和 NOD2 受体，其程度与大肠杆菌 BW25113 的胞壁肽相当。这些数据挑战了 PG 的非 N-乙酰化可用作 NOD-1 逃避机制的假设。我们的结果为关键肠道微生物群成员的细胞包膜结构的多样性及其在指导宿主-微生物组相互作用中的作用提供了新的见解。
Akkermansia muciniphilais an intestinal symbiont known to improve the gut barrier function in mice and humans. Various cell envelope components have been identified to play a critical role in the immune signaling ofA. muciniphila,but the chemical composition and role of peptidoglycan (PG) remained elusive. Here, we isolated PG fragments fromA. muciniphilaMucT(ATCC BAA-835),analyzed their composition and evaluated their immune signaling capacity. Structurally, the PG ofA. muciniphilawas found to be noteworthy due of the presence of some nonacetylated glucosamine residues, which presumably stems from deacetylation ofN-acetylglucosamine. Some of theN-acetylmuramic acid (MurNAc) subunits wereO-acetylated. The immunological assays revealed that muropeptides released from theA. muciniphilaPG could both activate the intracellular NOD1 and NOD2 receptors to a comparable extent as muropeptides fromEscherichia coliBW25113. These data challenge the hypothesis that non-N-acetylattion of PG can be used as a NOD-1 evasion mechanism. Our results provide new insights into the diversity of cell envelope structures of key gut microbiota members and their role in steering host–microbiome interactions.