Exposure to Colony Stimulating Factor 2 During Preimplantation Development Increases Postnatal Growth in Cattle
Exposure to Colony Stimulating Factor 2 During Preimplantation Development Increases Postnatal Growth in Cattle
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DOI：
10.1002/mrd.22533
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发表时间：
2015-11-01
影响因子：
2.5
通讯作者：
Hansen, Peter J.
中科院分区：
文献类型：
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作者：
Kannampuzha-Francis, Jasmine;Denicol, Anna C.;Hansen, Peter J.
植入前胚胎的微环境可以影响发育的变化，从而影响出生后的表型。在许多物种中，这种效应的潜在介体之一是集落刺激因子(CSF2)，它可以提高胚胎移植到受者体内后建立妊娠的能力。胚胎在发育早期暴露于CSF2也会以性别相关的方式影响怀孕后期的发育模式。因此，我们假设，在培养中使用CSF2处理体外产生的胚胎将改变所产生的小牛的出生体重和出生后的生长。测定了体外生产的荷斯坦小母牛在添加10 ng/mlCSF2和不添加10 ng/mlCSF2的情况下，以及通过人工授精生产的小母牛的体重和胸围。两组之间的出生体重没有差异；然而，此后，接受CSF2处理的牛犊在13个月龄期间体重增长更大，只有马肩隆身高的差异很小。这些结果支持这样的模型，即胚胎的出生后特征可以在植入前被编程，CSF2是通过编程来指导的胚胎因子之一。
The microenvironment of a preimplantation embryo can influence changes in development that affect postnatal phenotypes. One of the potential mediators of this effect in many species is colony-stimulating factor (CSF2), which can increase an embryo's ability to establish pregnancy after its transfer into recipients. Exposure of embryos to CSF2 during early development can also affect the pattern of development later in pregnancy in a sex-dependent manner. We therefore hypothesized that treatment of in vitro-produced embryos with CSF2 in culture would alter birth weight and postnatal growth of the resultant calf. Body weight and withers height were measured for Holstein heifer calves produced in vitro with or without 10 ng/ml CSF2 and for calves produced by artificial insemination. There were no differences in birth weight between groups; thereafter, however, calves from the CSF2-treated group experienced greater increases in body weight through 13 months of age, with only small differences in withers height. These results support the model that an embryo's postnatal characteristics can be programmed during the preimplantation period, and that CSF2 is one of the embryokines through which programming is directed.