Activation of macrophages microglia with the calcium-binding proteins MRP14 and MRP8 is related to the lesional activities in the spinal cord of HTLV-I associated myelopathy
Activation of macrophages microglia with the calcium-binding proteins MRP14 and MRP8 is related to the lesional activities in the spinal cord of HTLV-I associated myelopathy
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DOI：
10.1007/s004150050363
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发表时间：
1999-05-01
影响因子：
6
通讯作者：
Osame, M
中科院分区：
文献类型：
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作者：
Abe, M;Umehara, F;Osame, M
巨噬细胞和小胶质细胞可能在HTLV-I相关性脊髓病（HAM）和热带痉挛性截瘫（TSP）的慢性炎症过程中发挥重要作用。然而，HAM/TSP慢性炎症的病因和细胞机制尚不清楚，为了帮助确定巨噬细胞和小胶质细胞的作用，我们使用几种识别不同激活状态的单克隆抗体分析了HAM/TSP中枢神经系统（CNS）中巨噬细胞和小胶质细胞的各种激活模式。结果表明，在病程短（2.5年）的患者中，大量巨噬细胞和小胶质细胞同时表达MRCP14和MRP8非活动性慢性炎性病变。在患病时间为4.5年的患者中，血管周围和小胶质细胞周围的巨噬细胞对MRP8有反应，但对NIRP14没有反应。相比之下，MRP14和MRP8在非活动性慢性病变和对照组的血管周围和实质巨噬细胞和小胶质细胞上呈阴性。本研究提示：(a)活化的巨噬细胞和小胶质细胞以及CD4(+) T淋巴细胞和CD8（+）细胞毒性T淋巴细胞是HAM/TSP中枢神经系统炎症过程的主要成分；(b)活化的巨噬细胞和小胶质细胞与原位HTLV-I前病毒DNA的数量有关。
Macrophages and microglia may play an important role in the pathogenesis of chronic inflammatory process in HTLV-I associated myelopathy (HAM) and tropical spastic paraparesis (TSP). However, the etiology and cellular mechanism of chronic inflammation are poorly understood in HAM/TSP,To help to define the roles of macrophages and microglia we analyzed the various patterns of macrophage and microglia activation in the central nervous system (CNS) of HAM/TSP using several monoclonal antibodies recognizing the different states of activation. The results indicate that a large number of macrophages and microglia express both MRCP14 and MRP8 inactive-chronic inflammatory lesions of the patients with a short duration of illness (2.5 years). In the patient whose duration of illness was 4.5 years, perivascular and parenchymal macrophages rind microglia were reactive for MRP8 but not for NIRP14. In contrast, MRP14 and MRP8 were negative on the perivascular and parenchymal macrophages and microglia in inactive-chronic lesions and in controls. This study suggests that (a) activated macrophages and microglia as well as CD4(+) T lymphocytes and CD8(+) cytotoxic T lymphocytes are main components of the inflammatory process in the CNS in HAM/TSP, (b) activation of macrophages and microglia is related to the amount of HTLV-I proviral DNA in situ.