Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans
Morphologically distinct microtubule ends in the mitotic centrosome of Caenorhabditis elegans
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DOI：
10.1083/jcb.200304035
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发表时间：
2003-11-10
影响因子：
7.8
通讯作者：
Müller-Reichert, T
中科院分区：
文献类型：
--
作者：
O'Toole, ET;McDonald, KL;Müller-Reichert, T
在有丝分裂过程中，微管与中心体和着丝点的连接是动态的。从体外研究中，我们知道MT的动态行为与其末端的结构有关，但我们对纺锤体中MT末端的结构知之甚少。在这里，我们使用高压电子断层扫描研究了秀丽隐杆线虫胚胎细胞中纺锤体mt的中心体和着丝点相关末端。中心体相关的MT端要么闭合要么开放。封闭的MT端数量更多，并且均匀分布在中心体周围，而开放的MT端则优先出现在着丝酶附着的MT上。这些结果对MT与中心体相互作用的模型具有结构意义。
During mitosis, the connections of microtubules (MTs) to centrosomes and kinetochores are dynamic. From in vitro studies, it is known that the dynamic behavior of MTs is related to the structure of their ends, but we know little about the structure of MT ends in spindles. Here, we use high-voltage electron tomography to study the centrosome- and kinetochore-associated ends of spindle MTs in embryonic cells of the nematode, Caenorhabditis elegans. Centrosome-associated MT ends are either closed or open. Closed MT ends are more numerous and are uniformly distributed around the centrosome, but open ends are found preferentially on kinetochore-attached MTs. These results have structural implications for models of MT interactions with centrosomes.