Activation of H+-ATPase of the plasma membrane of Saccharomyces cerevisiae by glucose: the role of sphingolipid and lateral enzyme mobility.
Activation of H+-ATPase of the plasma membrane of Saccharomyces cerevisiae by glucose: the role of sphingolipid and lateral enzyme mobility.
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DOI：
10.1371/journal.pone.0030966
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Valiakhmetov A
中科院分区：
文献类型：
--
作者：
Permyakov S;Suzina N;Valiakhmetov A
葡萄糖对酿酒酵母质膜H+-ATP酶的激活是一个复杂的过程，至今尚未完全阐明。本研究旨在阐明脂质的作用和酶复合物的横向流动性在其激活葡萄糖。利用鞘脂和麦角甾醇合成受到干扰的菌株lcb 1 -100和erg 6，证明了H+-ATPase寡聚化对葡萄糖激活H+-ATPase的重要性。GFP融合的H+-ATP酶的实验表明，在葡萄糖活化过程中，荧光各向异性降低，这表明分子结构域的结构重组。免疫金分析表明，与葡萄糖孵育的结果在质膜中的ATP酶复合物的空间重新分布。结果表明：（1）H ~+-ATP酶被葡萄糖激活时处于寡聚状态;（2）葡萄糖激活时伴随着质膜H ~+-ATP酶簇的空间运动。
Activation of the plasma membrane H+-ATPase of the yeast Saccharomyces cerevisiae by glucose is a complex process that has not yet been completely elucidated. This study aimed to shed light on the role of lipids and the lateral mobility of the enzyme complex during its activation by glucose. The significance of H+-ATPase oligomerization for the activation of H+-ATPase by glucose was shown using the strains lcb1-100 and erg6, with the disturbed synthesis of sphyngolipid and ergosterol, respectively. Experiments with GFP-fused H+-ATPase showed a decrease in fluorescence anisotropy during the course of glucose activation, suggesting structural reorganization of the molecular domains. An immunogold assay showed that the incubation with glucose results in the spatial redistribution of ATPase complexes in the plasma membrane. The data suggest that (1) to be activated by glucose, H+-ATPase is supposed to be in an oligomeric state, and (2) glucose activation is accompanied by the spatial movements of H+-ATPase clusters in the PM.
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DOI：
10.1083/jcb.140.4.821
发表时间：
1998-02-23
期刊：
The Journal of cell biology
影响因子：
--
作者：
Partikian A;Olveczky B;Swaminathan R;Li Y;Verkman AS
通讯作者：
Verkman AS
影响因子：
2.9
作者：
LUTSENKO, S;KAPLAN, JH
通讯作者：
KAPLAN, JH
影响因子：
64.8
作者：
Auer, M;Scarborough, GA;Kühlbrandt, W
通讯作者：
Kühlbrandt, W
影响因子：
5.4
作者：
Campetelli, AN;Previtali, G;Casale, CH
通讯作者：
Casale, CH
影响因子：
4
作者：
Malinska, K;Malinsky, J;Tanner, W
通讯作者：
Tanner, W