Is premeiotic genome elimination an exclusive mechanism for hemiclonal reproduction in hybrid males of the genus Pelophylax?
Is premeiotic genome elimination an exclusive mechanism for hemiclonal reproduction in hybrid males of the genus Pelophylax?
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DOI：
10.1186/s12863-016-0408-z
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发表时间：
2016-07-02
期刊：
影响因子：
2.9
通讯作者：
Choleva L
中科院分区：
文献类型：
--
作者：
Doležálková M;Sember A;Marec F;Ráb P;Plötner J;Choleva L
从真核生殖细胞中消除亲本基因组的能力是一种主要在显示有性生殖的替代繁殖的杂交生物中观察到的现象。对于大多数分类群，基本的细胞途径和消除过程的时间仅知之甚少。在水蛙杂交种Pelophylax esculentus（亲本类群是P. ridibundus和P. lessonae）中，唯一描述的机制假设在变态期间和减数分裂之前，一个亲本基因组被排除在种系之外，而只有第二个基因组在核内复制后进入减数分裂。我们的研究杂种从P. ridiatrius-P. esculentus雄性群体的生产更多类型的配子已知的显示，杂交遗传机制的基因组消除是不统一的。利用比较基因组杂交（CGH）的有丝分裂和减数分裂细胞阶段，我们确定了至少两个途径的减数分裂机制。一种类型的Pelophylax esculentus男性提供了支持的证据，减数分裂前消除一个父母的基因组。在其他几个雄性中，我们记录了在减数分裂前期I（双线期）和中期I的晚期阶段双亲基因组的存在。一些油鲶雄性在减数分裂之前没有从生殖系中消除基因组。考虑到以前的细胞学和实验证据ridibundus和lessonae精子形成在一个单一的P. esculentus个人，我们提出了一个假设，即基因组消除从种系可以推迟到减数分裂阶段或完全没有在这些杂种。本文的在线版本（doi：10.1186/s12863-016-0408-z）包含补充材料，可供授权用户使用。
The ability to eliminate a parental genome from a eukaryotic germ cell is a phenomenon observed mostly in hybrid organisms displaying an alternative propagation to sexual reproduction. For most taxa, the underlying cellular pathways and timing of the elimination process is only poorly understood. In the water frog hybrid Pelophylax esculentus (parental taxa are P. ridibundus and P. lessonae) the only described mechanism assumes that one parental genome is excluded from the germline during metamorphosis and prior to meiosis, while only second genome enters meiosis after endoreduplication. Our study of hybrids from a P. ridibundus—P. esculentus-male populations known for its production of more types of gametes shows that hybridogenetic mechanism of genome elimination is not uniform. Using comparative genomic hybridization (CGH) on mitotic and meiotic cell stages, we identified at least two pathways of meiotic mechanisms. One type of Pelophylax esculentus males provides supporting evidence of a premeiotic elimination of one parental genome. In several other males we record the presence of both parental genomes in the late phases of meiotic prophase I (diplotene) and metaphase I. Some P. esculentus males have no genome elimination from the germ line prior to meiosis. Considering previous cytological and experimental evidence for a formation of both ridibundus and lessonae sperm within a single P. esculentus individual, we propose a hypothesis that genome elimination from the germline can either be postponed to the meiotic stages or absent altogether in these hybrids. The online version of this article (doi:10.1186/s12863-016-0408-z) contains supplementary material, which is available to authorized users.