1024-Pixel CMOS Multimodality Joint Cellular Sensor/Stimulator Array for Real-Time Holistic Cellular Characterization and Cell-Based Drug Screening.
1024-Pixel CMOS Multimodality Joint Cellular Sensor/Stimulator Array for Real-Time Holistic Cellular Characterization and Cell-Based Drug Screening.
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DOI：
10.1109/tbcas.2017.2759220
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发表时间：
2018-03
影响因子：
5.1
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Park JS;Aziz MK;Li S;Chi T;Grijalva SI;Sung JH;Cho HC;Wang H
本文提出了一种完全集成的CMOS多模态联合传感器/刺激器阵列与1024像素的实时整体细胞表征和药物筛选。该系统由四个像素组和四个并行信号调节模块组成。每个像素组包含16 × 16个像素，每个像素包括一个28 μm × 28 μm镀金电极、四个12 μm × 12 μm光电二极管和像素内电路，像素尺寸为58 μm × 58 μm。每个像素支持实时细胞外电位记录、光学检测、电荷平衡双相电流刺激和相同细胞样本的细胞阻抗测量。该系统是在一个标准的130 nm CMOS工艺制造。大鼠心肌细胞在芯片上培养成功。测量的高分辨率光学不透明度图像，细胞外电位记录，双相电流刺激和细胞阻抗图像证明了该系统在整体细胞表征和药物筛选方面的独特优势。此外，本文演示了使用光学检测的芯片上培养的心肌细胞，实时跟踪他们的周期性搏动模式和搏动率。
This paper presents a fully integrated CMOS multimodality joint sensor/stimulator array with 1024 pixels for real-time holistic cellular characterization and drug screening. The proposed system consists of four pixel groups and four parallel signal-conditioning blocks. Every pixel group contains 16 × 16 pixels, and each pixel includes one 28 μm × 28 μm gold-plated electrode, four 12 μm × 12 μm photodiodes, and in-pixel circuits, within a 58 μm × 58 μm pixel footprint. Each pixel supports real-time extracellular potential recording, optical detection, charge-balanced biphasic current stimulation, and cellular impedance measurement for the same cellular sample. The proposed system is fabricated in a standard 130-nm CMOS process. Rat cardiomyocytes are successfully cultured on-chip. Measured high-resolution optical opacity images, extracellular potential recordings, biphasic current stimulations, and cellular impedance images demonstrate the unique advantages of the system for holistic cell characterization and drug screening. Furthermore, this paper demonstrates the use of optical detection on the on-chip cultured cardiomyocytes to real-time track their cyclic beating pattern and beating rate.