SPHERE-ROD MORPHOGENESIS OF ESCHERICHIA-COLI
SPHERE-ROD MORPHOGENESIS OF ESCHERICHIA-COLI
复制标题
DOI：
10.1099/00221287-86-2-201
复制
发表时间：
1975-01-01
期刊：
影响因子：
--
通讯作者：
SCHWARZ, U
中科院分区：
文献类型：
--
作者：
GOODELL, EW;SCHWARZ, U
e的形态发生能力。大肠杆菌的研究是通过将杆状细胞转化为球体，然后确定这些球体是否可以恢复为杆状细胞。将细胞固定在含有methocel的粘性培养基中，使细胞形成。制备了两种不同类型的球体：保留机械完整的球囊的细胞和缺乏球囊的渗透敏感的球质体。球质体虽能合成一个新的球囊，但不能恢复为杆状体。相比之下，保留完整球囊的球体能够将自己重塑成棒状。它们还能够在原杆末端的位置（或附近）形成新的末端。
The morphogenetic capacity ofE. coliwas studied by converting the rod-shaped cells into spheres and then determining whether these spheres could revert to rods. The morphogenesis of cells was followed by immobilizing them in a viscous Methocel-containing medium. Two different types of spheres were prepared: cells which retained a mechanically intact sacculus, and osmotically sensitive sphaeroplasts lacking a sacculus. The sphaeroplasts were not able to revert to rods although they were able to synthesize a new sacculus. In contrast, spheres which had retained an intact sacculus were able to reshape themselves into rods. They were also able to form new ends at (or near) the sites of the ends on the original rods.