Characterisation of the Pacific Oyster Microbiome During a Summer Mortality Event
Characterisation of the Pacific Oyster Microbiome During a Summer Mortality Event
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DOI：
10.1007/s00248-018-1226-9
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发表时间：
2019-02-01
影响因子：
3.6
通讯作者：
Labbate, Maurizio
中科院分区：
文献类型：
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作者：
King, William L.;Jenkins, Cheryl;Labbate, Maurizio
太平洋牡蛎Crassostrea gigas是全球养殖的重要商业物种。近年来，疾病爆发严重影响了C。世界各地的gigas库存，在夏季发生了许多损失。许多传染性病原体与这些夏季死亡事件有关，包括病毒（特别是Ostreid疱疹病毒1，OsHV-1）和细菌;然而，无法确定已知病原体的病例很常见。在这项研究中，我们检查了受疾病影响和未受疾病影响的C。在2013-2014年夏季斯蒂芬斯港（澳大利亚）的死亡事件中，没有检测到包括OsHV-1在内的已知牡蛎病原体。70 ℃时闭壳肌微生物群的变化。使用16 S rRNA（V1-V3区域）扩增子测序对斯蒂芬斯港河口12个研究地点的gigas样本进行了表征，目的是比较空间位置和疾病状态对牡蛎微生物组的影响。研究发现，空间位置是受疾病影响的牡蛎微生物组的重要决定因素。此外，疾病状态之间的微生物组比较发现，在受疾病影响的微生物组中，属于哈氏弧菌的罕见操作分类单位（OTU）和弧菌属的未识别成员显著增加。这表明弧菌在牡蛎疾病中的潜在作用，并支持以前对这种死亡事件进行的基于培养的检查。
The Pacific oyster, Crassostrea gigas, is a key commercial species that is cultivated globally. In recent years, disease outbreaks have heavily impacted C. gigas stocks worldwide, with many losses incurred during summer. A number of infectious agents have been associated with these summer mortality events, including viruses (particularly Ostreid herpesvirus 1, OsHV-1) and bacteria; however, cases where no known aetiological agent can be identified are common. In this study, we examined the microbiome of disease-affected and disease-unaffected C. gigas during a 2013-2014 summer mortality event in Port Stephens (Australia) where known oyster pathogens including OsHV-1 were not detected. The adductor muscle microbiomes of 70 C. gigas samples across 12 study sites in the Port Stephens estuary were characterised using 16S rRNA (V1-V3 region) amplicon sequencing, with the aim of comparing the influence of spatial location and disease state on the oyster microbiome. Spatial location was found to be a significant determinant of the disease-affected oyster microbiome. Furthermore, microbiome comparisons between disease states identified a significant increase in rare operational taxonomic units (OTUs) belonging to Vibrio harveyi and an unidentified member of the Vibrio genus in the disease-affected microbiome. This is indicative of a potential role of Vibrio species in oyster disease and supportive of previous culture-based examination of this mortality event.