Molecular detection and genetic characteristics of Giardia duodenalis in dairy cattle from large-scale breeding farms in Xinjiang, China
Molecular detection and genetic characteristics of Giardia duodenalis in dairy cattle from large-scale breeding farms in Xinjiang, China
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DOI：
10.1007/s00436-024-08123-2
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发表时间：
2024-01-01
影响因子：
2
通讯作者：
Zhang，Zhenjie
中科院分区：
文献类型：
--
作者：
Zhao，Qianming;Yang，Bin;Zhang，Zhenjie
十二指肠贾第鞭毛虫是一种肠道原生动物，可以感染人类和动物，导致公共卫生问题和畜牧业的经济损失。据报道，十二指肠杆菌感染了奶牛，但有关中国新疆大型奶牛场的信息有限。该研究从中国新疆的五个大型养牛场收集了 749 份新鲜粪便样本。该研究使用小亚基核糖体 RNA (SSU rRNA*) 基因的巢式 PCR 检测来确定 G 的存在。结果显示，24.0%（180/749）的奶牛G呈阳性。十二指肠球菌，在断奶前犊牛中观察到的感染率最高 (45.1%, 69/153)。 180G之中。十二指肠球菌阳性样本中，鉴定出三个组合：组合 E（n= 176）、组合 A（n= 3）和组合 B（n= 1）。分别获得了β-贾第素(bg*)基因、谷氨酸脱氢酶(gdh*)基因和磷酸丙糖异构酶(tpi*)基因的69、67和49个序列。鉴定了组合 E 的 13 个新序列，包括来自 bg* 基因的 5 个序列、来自 gdh* 基因的 4 个序列和来自 tpi* 基因的 4 个序列。本次研究发现32G。十二指肠组合 E 分离株形成 26 个 MLG，表明牛源 G 中存在遗传变异和基于地理隔离的分化。这些发现为 G 的遗传多样性提供了基本的见解。十二指肠球菌素可用于规模化奶牛养殖场的预防和控制。
Giardia duodenalisis an intestinal protozoan that can infect both humans and animals, leading to public health issues and economic losses in the livestock industry.G. duodenalishas been reported to infect dairy cattle, but there is limited information available on large-scale dairy farms in Xinjiang, China. The study collected 749 fresh faecal samples from five large-scale cattle farms in Xinjiang, China. The study used a nested PCR assay of the small subunit ribosomal RNA (SSU rRNA*) gene to determine the presence ofG. duodenalis.The results showed that 24.0% (180/749) of dairy cattle were positive forG. duodenalis, with the highest infection rate observed in pre-weaned calves (45.1%, 69/153). Among the 180G. duodenalispositive samples, three assemblages were identified: assemblage E (n= 176), assemblage A (n= 3) and assemblage B (n= 1). Sixty-nine, 67 and 49 sequences were obtained for the beta-giardin (bg*) gene, the glutamate dehydrogenase (gdh*) gene and the triose phosphate isomerase (tpi*) gene, respectively. Thirteen novel sequences of assemblage E were identified, including five sequences from the bg* gene, four sequences from the gdh* gene and four sequences from the tpi* gene. This study found that 32G. duodenalisassemblage E isolates formed 26 MLGs, indicating genetic variation and geographic isolation-based differentiation in bovine-derivedG. duodenalisassemblage E. These findings provide fundamental insights into the genetic diversity ofG. duodenalisin dairy cattle and can aid in the prevention and control of its occurrence in large-scale dairy cattle farms.