Bacillus licheniformis escapes from Myxococcus xanthus predation by deactivating myxovirescin A through enzymatic glucosylation.
Bacillus licheniformis escapes from Myxococcus xanthus predation by deactivating myxovirescin A through enzymatic glucosylation.
复制标题
DOI：
10.1111/1462-2920.14817
复制
发表时间：
2019-12
影响因子：
5.1
通讯作者：
Chuandong Wang;Xinli Liu;Peng Zhang;Yan Wang;Zhi-feng Li;Xun Li;Renqing Wang;Z. Shang;
中科院分区：
文献类型：
--
作者：
Chuandong Wang;Xinli Liu;Peng Zhang;Yan Wang;Zhi-feng Li;Xun Li;Renqing Wang;Z. Shang;
黄色粘球菌在捕食过程中使用粘病毒素A（TA）杀死易感细菌。然而，自然界中的猎物细胞是否能逃脱M。xanthus通过发展对TA的抗性是未知的。我们观察到，许多现场分离的地衣芽孢杆菌菌株可以生存遇到M。xanthus，这与它们的TA抗性相关。在抗捕食B的发酵液中鉴定出TA糖苷。地衣芽孢杆菌J32与M.添加TA后，J32的糖基转移酶基因（yjiC）表达上调。异源表达的YjiC通过将葡萄糖部分缀合至C-21羟基将TA修饰为TA糖苷（TA-Gluc），并且所得化合物与共培养液中存在的TA糖苷相同。TA-Gluc由于其与LspA的结合较弱而表现出降低的杀菌活性，如计算机对接数据所示。yjiC基因的异源表达赋予TA和M。对寄主E. coli细胞。此外，在捕食压力下，B.地衣菌Y 071通过yjiC和lspA基因的过表达获得TA抗性，从而迅速发展出捕食抗性。这些结果表明M. B的抗捕食性。地衣状芽孢杆菌是由于TA失活的葡萄糖基化，这是诱导捕食者介导的方式。本文受版权保护。All rights reserved.
Myxococcus xanthus kills susceptible bacteria using myxovirescin A (TA) during predation. However, whether prey cells in nature can escape M. xanthus by developing resistance to TA is unknown. We observed that many field-isolated Bacillus licheniformis strains could survive encounters with M. xanthus, which was correlated to their TA resistance. A TA glycoside was identified in the broth of predation-resistant B. licheniformis J32 cocultured with M. xanthus, and a glycosyltransferase gene (yjiC) was up-regulated in J32 after the addition of TA. Hetero-expressed YjiC modified TA to a TA glucoside (TA-Gluc) by conjugating a glucose moiety to the C-21 hydroxyl group, and the resulting compound was identical to the TA glycoside present in the coculture broth. TA-Gluc exhibited diminished bactericidal activity due to its weaker binding with LspA, as suggested by in silico docking data. Heterologous expression of the yjiC gene conferred both TA and M. xanthus-predation resistance to the host E. coli cells. Furthermore, under predatory pressure, B. licheniformis Y071 rapidly developed predation-resistance by acquiring TA-resistance through the overexpression of yjiC and lspA genes. These results suggest that M. xanthus predation resistance in B. licheniformis is due to the TA deactivation by glucosylation, which is induced in a predator-mediated manner. This article is protected by copyright. All rights reserved.