Production of 2-(2-phenylethyl)chromones in Aquilaria sinensis calli under different treatments
Production of 2-(2-phenylethyl)chromones in Aquilaria sinensis calli under different treatments
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不同处理下沉香愈伤组织2-(2-苯乙基)色酮的产生
DOI：
10.1007/s11240-018-1442-5
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发表时间：
2018-10-01
影响因子：
3
通讯作者：
Shi, Shepo
中科院分区：
文献类型：
--
作者：
Dong, Xianjuan;Gao, Bowen;Shi, Shepo
摘要 2-(2-苯乙基)色酮是从沉香中分离出来的主要芳香族化合物，沉香是来自沉香的树脂部分，用作传统药物、熏香和香水。然而，2-(2-苯乙基)色酮的生物合成和沉香形成的机制仍不清楚。前期研究表明，6,7-二甲氧基-2-[2-(4′-甲氧基苯基)乙基]色酮(AH8)和6,7-二甲氧基-2-(2-苯乙基)色酮(AH6)对盐胁迫更敏感，可作为分析2-(2-苯乙基)色酮产生的标记。本研究采用AB Sciex 5500 Qtrap质谱仪分析了AH6和AH8的含量，并采用LC-DAD-IT-TOF-MS系统检测了不同非生物胁迫和激素处理下的中华愈伤组织中2-(2-苯乙基)色酮的同系物。在非生物胁迫中，盐胁迫和干旱胁迫显着增加了AH6和AH8的产量，并分别诱导41种和23种2-(2-苯乙基)色酮。但冷处理对2-(2-苯乙基)色酮的生产影响不大。 KNO3、CaCl2 和 (NH4)2SO4 诱导的离子胁迫也显着增加了 AH6 和 AH8 的含量，并分别诱导了 14、18 和 14 个 2-(2-苯乙基)色酮同系物。外源激素MeJA、SA和ABA在20天内诱导愈伤组织中产生少量2-(2-苯乙基)色酮，而MeJA、SA和ABA处理后分别检测到6、5和7种2-(2-苯乙基)色酮。我们的研究将为进一步研究2-(2-苯乙基)色酮的生物合成和沉香的形成提供坚实的基础。
Abstract2-(2-Phenylethyl)chromones are main classes of aromatic compounds isolated from agarwood, the resinous portion derived from Aquilaria sinensis used as traditional medicine, incense and perfume. However, the mechanisms of biosynthesis of 2-(2-phenylethyl)chromones and agarwood formation are still unknown. Previous studies indicated that 6,7-dimethoxy-2-[2-(4′-methoxyphenyl)ethyl]chromone (AH8) and 6,7-dimethoxy-2-(2-phenylethyl)chromone (AH6) were more sensitive to salt stress and can be used as markers to analyze the production of 2-(2-phenylethyl)chromones. In this study, the content of AH6 and AH8 was analyzed by an AB Sciex 5500 Qtrap mass spectrometer and the congeners of 2-(2-phenylethyl)chromones were detected by LC-DAD-IT-TOF-MS system in A. sinensis calli under various abiotic stresses and hormone treatments. Among the abiotic stresses, salt and drought stress remarkably increased the production of AH6 and AH8, and could induce 41 and 23 species of 2-(2-phenylethyl)chromones, respectively. However, cold treatment has little influence on the production of 2-(2-phenylethyl)chromones. Ion stresses induced by KNO3, CaCl2, and (NH4)2SO4 also significantly increased the content of AH6 and AH8, and could induced 14, 18, and 14 congeners of 2-(2-phenylethyl)chromones, respectively. Exogenous hormones including MeJA, SA and ABA induced a small amount of 2-(2-phenylethyl)chromones in calli within 20 days, while 6, 5, and 7 species of 2-(2-phenylethyl)chromones were detected after MeJA, SA and ABA treatment, respectively. Our study would provide solid basis for further research on biosynthesis of 2-(2-phenylethyl)chromones and agarwood formation.