INACTIVATION OF CONTRACEPTIVE STEROID-HORMONES BY HUMAN INTESTINAL CLOSTRIDIA
INACTIVATION OF CONTRACEPTIVE STEROID-HORMONES BY HUMAN INTESTINAL CLOSTRIDIA
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DOI：
10.1128/jcm.18.3.500-504.1983
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发表时间：
1983-01-01
影响因子：
9.4
通讯作者：
MOSBACH, EH
中科院分区：
文献类型：
--
作者：
BOKKENHEUSER, VD;WINTER, J;MOSBACH, EH
在代谢实验中，人类粪便菌群将天然孕激素的β4-3-酮结构还原为3α-羟基，5β-类固醇代谢物(3α，5β。)以及合成孕激素到3α，5β的混合物。和3.beta、5.beta。化合物。3.α，5，β-还原酶是由副产气荚膜梭菌合成的，对孕酮等天然孕激素有很强的亲和力。3.β-，5-β-还原酶是由白头翁合成的，对合成的孕激素有较强的亲和力。圣卢克S菌株209(Clostridium sp.J-1)合成了第三种酶，3β，5α-还原酶，但只有在纯培养条件下才能观察到，合成孕酮A环的还原速度比天然孕激素慢3-10倍，这说明合成孕酮比天然孕激素具有药理上的优越性。
In metabolic experiments, human fecal flora reduced the .DELTA.4-3-keto structure of natural progestins to 3.alpha.-hydroxy, 5.beta.-steroid metabolites (3.alpha.,5.beta.) and of synthetic progestins to a mixture of 3.alpha.,5.beta. and 3.beta.,5.beta. compounds. 3.alpha.,5.beta.-Reductase was synthesized by Clostridium paraputrificum and had a strong affinity for natural progestins such as progesterone. 3.beta.,5.beta.-Reductase was synthesized by C. innocuum and had a stronger affinity for synthetic progestins. A 3rd enzyme, 3.beta.,5.alpha.-reductase, was synthesized by St. Luke''s strain 209 (Clostridium sp. J-1) but was only observed when pure cultures were used. Ring A reduction of synthetic progestins was 3-10 times slower than that of natural progestins, thus, explaining the pharmacological superiority of synthetic progestins over naturally occurring analogs.