In situ expression of eukaryotic ice-binding proteins in microbial communities of Arctic and Antarctic sea ice
In situ expression of eukaryotic ice-binding proteins in microbial communities of Arctic and Antarctic sea ice
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DOI：
10.1038/ismej.2015.43
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发表时间：
2015-11-01
期刊：
影响因子：
11
通讯作者：
Beszteri, Bank
中科院分区：
文献类型：
--
作者：
Uhlig, Christiane;Kilpert, Fabian;Beszteri, Bank
冰结合蛋白（IBP）已经从各种海冰生物中分离出来。它们的特征表明，在保护零度以下环境中的生物体方面发挥着至关重要的作用。然而，它们在天然海冰微生物群落中的原位丰度和多样性在很大程度上是未知的。在这项研究中，我们分析了真核生物IBP转录本的表达和系统发育多样性的微生物群落的北极和南极海冰。IBP成绩单被发现在丰度类似的核心细胞过程，如光合作用的蛋白质。89%的IBP转录本与来自硅藻、附着菌和甲壳类动物的已知IBP序列组合在一起，但大多数代表了以前在培养生物中未表征的新序列。在天然真核海冰群落中观察到的高真核IBP表达强调了IBP对于生活在极地海冰极端条件下的群落中许多微生物的生存的重要作用。
Ice-binding proteins (IBPs) have been isolated from various sea-ice organisms. Their characterisation points to a crucial role in protecting the organisms in sub-zero environments. However, their in situ abundance and diversity in natural sea-ice microbial communities is largely unknown. In this study, we analysed the expression and phylogenetic diversity of eukaryotic IBP transcripts from microbial communities of Arctic and Antarctic sea ice. IBP transcripts were found in abundances similar to those of proteins involved in core cellular processes such as photosynthesis. Eighty-nine percent of the IBP transcripts grouped with known IBP sequences from diatoms, haptophytes and crustaceans, but the majority represented novel sequences not previously characterized in cultured organisms. The observed high eukaryotic IBP expression in natural eukaryotic sea ice communities underlines the essential role of IBPs for survival of many microorganisms in communities living under the extreme conditions of polar sea ice.