Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats
Alloxan diabetes alters the tensile strength, morphological and morphometric parameters of abdominal wall healing in rats
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DOI：
10.1590/s0102-86502014000200008
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发表时间：
2014-02-01
期刊：
影响因子：
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通讯作者：
Spadella, César Tadeu
中科院分区：
文献类型：
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作者：
Minossi, José Guilherme;Lima, Flávio de Oliveira;Spadella, César Tadeu
目的：探讨四氧嘧啶糖尿病对开腹大鼠腹壁愈合的影响。方法：Wistar雄性大鼠96只，体重200~300g，随机分为非糖尿病组(G1)和糖尿病未治疗组(G2)。糖尿病诱导3个月后，动物接受5 cm长的剖腹手术和5.0尼龙单丝缝合。术后第4、14、21、30天每组各处死12只动物，分别取各时间点M1、M2、M3、M4处死。每隔1分钟取出一块含有疤痕的腹壁，用于抗拉强度测量、组织学和形态计量学研究。结果：G2组动物表现出与严重糖尿病和降低血浆胰岛素水平相适应的参数。G2的抗拉强度在M2和M4明显较小，其他两个有下降的趋势。光镜下观察，糖尿病大鼠更难增加胶原密度和收缩。G2组愈合后期成纤维细胞密度较高，M2、M4区胶原变薄。结论：糖尿病大鼠腹部创面愈合不良，裂开、感染发生率高。
PURPOSE: To investigate the effects of alloxan diabetes on the abdominal wall healing of rats undergoing laparotomy.METHODS: Ninety-six male Wistar rats weighing between 200 and 300 grams, divided into two groups: non-diabetic group (G1) and another with untreated diabetes (G2). Three months after diabetes induction, the animals underwent a 5cm-long-laparotomy and 5.0 nylon monofilament suture. After the surgery, 12 animals from each group were euthanized on days 4, 14, 21 and 30 corresponding to the moments M1, M2, M3 and M4. In each moment a fragment of the abdominal wall containing the scar was removed for tensile strength measurement, histological and morphometric study. Clinical and biochemical parameters were also analyzed.RESULTS: G2 animals showed parameters compatible with severe diabetes and decreased plasma levels of insulin. The tensile strength in G2 was significantly smaller in M2 and M4, with a tendency to fall in the other two. Through light microscope, diabetic animals showed more difficulty to increase collagen density and contraction. G2 animals showed high cellularity of fibroblasts in later healing moments, with collagen thinning in M2 and M4.CONCLUSION: The abdominal wound healing in untreated diabetic animals was altered and led to a higher incidence of dehiscence and infections.