A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis.
A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis.
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DOI：
10.1186/s12868-015-0183-1
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发表时间：
2015-07-24
期刊：
影响因子：
2.4
通讯作者：
Giudetti AM
中科院分区：
文献类型：
--
作者：
Vergara D;D'Alessandro M;Rizzello A;De Riccardis L;Lunetti P;Del Boccio P;De Robertis F;Trianni G;Maffia M;Giudetti AM
脂质在中枢神经系统中发挥着不同的重要作用，因此脂质通路的失调已经涉及越来越多的神经退行性疾病，包括多发性硬化症（MS）。MS是中枢神经系统最普遍的自身免疫性疾病，具有由炎症和脱髓鞘引起的神经系统症状。在本研究中，以非靶向方法对MS患者和对照样品的CD 4 + T淋巴细胞的快速谱进行脂质组学分析。采用基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱法，以9-氨基吖啶为基质，对脂质提取物进行分析。以负模式分析脂质，并使用MALDI串联质谱法对所选物质进行片段化以进行结构归属。该分析揭示了MS CD 4 + T淋巴细胞的磷脂模式相对于健康对照的一些修改，其中MS样品中的心磷脂种类显著增加。这些结果证明了MALDI-TOF方法用于分析CD 4+脂质提取物的可行性，并表明MS患者淋巴细胞的脂质代谢特征的改变。
Lipids play different important roles in central nervous system so that dysregulation of lipid pathways has been implicated in a growing number of neurodegenerative disorders including multiple sclerosis (MS). MS is the most prevalent autoimmune disorder of the central nervous system, with neurological symptoms caused by inflammation and demyelination. In this study, a lipidomic analysis was performed for the rapid profile of CD4+ T lymphocytes from MS patient and control samples in an untargeted approach. A matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry based approach was used for the analysis of lipid extracts using 9-aminoacridine as matrix. Lipids were analyzed in negative mode and selected species fragmented using MALDI tandem mass spectrometry for their structural assignments. The analysis reveals some modifications in the phospholipid pattern of MS CD4+ T lymphocytes with respect to healthy controls with a significant increase of cardiolipin species in MS samples. These results demonstrate the feasibility of a MALDI-TOF approach for the analysis of CD4+ lipid extracts and suggest how alterations in the lipid metabolism characterized lymphocytes of MS patients.