A cryptic wheat-Aegilops triuncialis translocation with leaf rust resistance gene Lr58
A cryptic wheat-Aegilops triuncialis translocation with leaf rust resistance gene Lr58
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DOI：
10.2135/cropsci2007.01.0038
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发表时间：
2007-09-01
期刊：
影响因子：
2.3
通讯作者：
Gill, Bikram S.
中科院分区：
文献类型：
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作者：
Kuraparthy, Vasu;Sood, Shilpa;Gill, Bikram S.
从野生物种转移到作物植物的基因通常与有害性状有关。利用分子标记技术，检测到一个来自山羊草的抗叶锈病基因的隐性渗入。普通小麦（Triticurn aestivum L.）从六倍体小麦与小麦品种A. triuncialis。原位杂交采用A. triuncialis基因组DNA作为探针未能检测到外源基因渗入。该易位系对印度和堪萨斯最流行的叶锈病小种具有抗性。在分离的F-2：3群体中进行的遗传作图表明，抗锈性为单基因遗传。同源异型群2限制性片段长度多态性标记XksuF 11、XksuH 16和Xbg 123在抗性和敏感群体之间显示出诊断多态性等位基因。外源基因转移起源于同源染色体重组。助理XksuH 16、XksuF 11、Xbg 123和一个简单重复序列标记Xcfd 50与小麦抗锈性共分离，表明小麦-A. triuncialis易位发生在染色体臂2BL的远端区域。该易位被命名为T2BS.2BL-2（t）L（0.95）。在213号染色体上的渗入的独特来源和图位表明，叶锈病抗性基因是新的，并被命名为Lr 58。
Genes transferred to crop plants from wild species are often associated with deleterious traits. Using molecular markers, we detected a cryptic introgression with a leaf rust resistance gene transferred from Aegilops triuncialis L. into common wheat (Triticurn aestivum L.). One agronomically desirable rust-resistant introgression line was selected and advanced to BC3F11 from a cross of hexaploid wheat and A. triuncialis. In situ hybridization using A. triuncialis genomic DNA as a probe failed to detect the alien introgression. The translocation line was resistant to the most prevalent races of leaf rust in India and Kansas. Genetic mapping in a segregating F-2:3 population showed that the rust resistance was monogenically inherited. Homeologous group 2 restriction fragment length polymorphism markers XksuF11, XksuH16, and Xbg123 showed diagnostically polymorphic alleles between the resistant and susceptible bulks. The alien transfer originated from homeologous chromosome recombination. The A. triuncialis-specific alleles of XksuH16, XksuF11, Xbg123, and one simple sequence repeat marker Xcfd50 cosegregated with the rust resistance, suggesting that the wheat-A. triuncialis translocation occurred in the distal region of chromosome arm 2BL. This translocation was designated T2BS.2BL-2(t)L(0.95). The unique source and map location of the introgression on chromosome 213 indicated that the leaf rust resistance gene is new and was designated Lr58.