The functional capabilities of cells leaving the thymus.
The functional capabilities of cells leaving the thymus.
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DOI：
10.4049/jimmunol.132.1.25
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发表时间：
1984-01
影响因子：
4.4
通讯作者：
R. Scollay;W. F. Chen;K. Shortman
中科院分区：
文献类型：
--
作者：
R. Scollay;W. F. Chen;K. Shortman
多年来，关于离开胸腺（胸腺移行细胞）进入外周淋巴器官的细胞的功能状态一直存在争议。它们是像皮质胸腺细胞一样免疫功能不全，因此可能是从皮质胸腺细胞衍生而来的，还是像髓质胸腺细胞一样功能成熟？直到最近，用于评估假定的胸腺移民的技术一直是间接的，但现在可以直接测量最近的胸腺移民的功能。我们用异硫氰酸荧光素溶液的胸腺内注射标记胸腺细胞，并使用电子细胞分选纯化的荧光细胞聚集在周围在以下3至4 hr. The移民已富集从原来的频率约1：1000在淋巴结和脾脏，大于98%的纯度。这些细胞已与正常外周T细胞的增殖和细胞毒性前体活性进行了比较，在高克隆效率，凝集素诱导，有限稀释培养系统和在同种异体有限稀释系统。增殖性淋巴细胞和细胞毒性淋巴细胞的前体的频率和产生的克隆的大小对于最近的移民和外周T细胞是相同的。因此，根据对有丝分裂原的增殖和细胞毒性反应以及产生同种异体特异性CTL的标准，胸腺移民在从胸腺移民后几个小时就具有完全的免疫活性;因此，我们没有看到任何证据表明胸腺后胸腺细胞类型的细胞在离开胸腺后需要主要的成熟步骤。
There has been a controversy for many years over the functional status of cells that leave the thymus (thymus migrants) to populate the peripheral lymphoid organs. Are they immunoincompetent like cortical thymocytes and so probably derived from them, or are they functionally mature like medullary thymocytes? Until recently the techniques used to assess putative thymus migrants have been indirect, but it is now possible to measure the function of recent thymus migrants directly. We used intrathymic injection of a solution of fluorescein isothiocyanate to label thymocytes, and used electronic cell sorting to purify the fluorescent cells that accumulate in the periphery over the following 3 to 4 hr. The migrants have been enriched from an original frequency of about 1:1000 in lymph nodes and spleen, to greater than 98% purity. These cells have been compared with normal peripheral T cells for proliferative and cytotoxic precursor activity in a high cloning efficiency, lectin-induced, limit dilution culture system and in an allospecific limit dilution system. The frequency of precursors of proliferative lymphocytes and cytotoxic lymphocytes and the size of the clones produced is the same for recent migrants and peripheral T cells. Thus by the criteria of proliferation and cytotoxic responses to mitogens and generation of allospecific CTL, thymus migrants, a few hours after their emigration from the thymus, are fully immunocompetent; we therefore see no evidence of a post-thymic precursor-type cell that requires major maturation steps after leaving the thymus.