X-ray crystallography reveals a reduced substrate complex of UDP-galactopyranose mutase poised for covalent catalysis by flavin.
X-ray crystallography reveals a reduced substrate complex of UDP-galactopyranose mutase poised for covalent catalysis by flavin.
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X射线晶体学揭示了黄素有益于共价催化的UDP-半乳吡喃糖突变酶的底物复合物减少。
DOI：
10.1021/bi901437v
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发表时间：
2009-10-06
期刊：
影响因子：
2.9
通讯作者：
Forest, Katrina T.
中科院分区：
文献类型：
--
作者：
Gruber, Todd D.;Westler, William M.;Kiessling, Laura L.;Forest, Katrina T.
The flavoenzyme uridine 5’-diphosphate galactopyranose mutase (UGM or Glf) catalyzes the interconversion of UDP-galactopyranose and UDP-galactofuranose. The latter is a key building block for cell wall construction in numerous pathogens, including Mycobacterium tuberculosis. Mechanistic studies of UGM suggested a novel role for the flavin, and we previously provided evidence that the catalytic mechanism proceeds through a covalent flavin-galactose iminium. Here, we describe 2.3 and 2.5 Å resolution X-ray crystal structures of the substrate-bound enzyme in oxidized and reduced forms, respectively. In the latter, the substrate C1 is 3.6 Å from the nucleophilic flavin N5 position. This orientation is consistent with covalent catalysis by flavin.
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影响因子：
5.6
作者：
Beis, K;Srikannathasan, V;Liu, H;Fullerton, SWB;Bamford, VA;Sanders, DAR;Whitfield, C;McNeil, MR;Naismith, JH
通讯作者：
Naismith, JH
影响因子：
2.9
作者：
Chad, Jennifer M.;Sarathy, Karunan Partha;Sanders, David A. R.
通讯作者：
Sanders, David A. R.
影响因子：
3.2
作者：
Pan, F;Jackson, M;McNeil, M
通讯作者：
McNeil, M
影响因子：
--
作者：
Schmalhorst, Philipp S.;Krappmann, Sven;Routier, Francoise H.
通讯作者：
Routier, Francoise H.
影响因子：
15
作者：
Yuan, Yue;Bleile, Dustin W.;Pinto, B. Mario
通讯作者：
Pinto, B. Mario