HIGH-RESOLUTION CONFORMATION OF GRAMICIDIN-A IN A LIPID BILAYER BY SOLID-STATE NMR
HIGH-RESOLUTION CONFORMATION OF GRAMICIDIN-A IN A LIPID BILAYER BY SOLID-STATE NMR
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DOI：
10.1126/science.7690158
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发表时间：
1993-09-10
期刊：
影响因子：
56.9
通讯作者：
CROSS, TA
中科院分区：
文献类型：
--
作者：
KETCHEM, RR;HU, W;CROSS, TA
用均匀排列的甘草素A脂双层制剂的固体核磁共振波谱，仅根据氨基酸序列和144个取向约束，解释了阳离子通道构象的高分辨二聚体结构。这个初始结构将螺旋间距定义为单链，将每转的残基数量固定在6到7，将螺旋感觉指定为右手，并识别氢键。这种初始结构的改进产生了合理的氢键距离，而扭转角只有很小的变化。
Solid-state nuclear magnetic resonance spectroscopy of uniformly aligned preparations of gramicidin A in lipid bilayers has been used to elucidate a high-resolution dimeric structure of the cation channel conformation solely on the basis of the amino acid sequence and 144 orientational constraints. This initial structure defines the helical pitch as single-stranded, fixes the number of residues per turn at six to seven, specifies the helix sense as right-handed, and identifies the hydrogen bonds. Refinement of this initial structure yields reasonable hydrogen-bonding distances with only minimal changes in the torsion angles.