A GAS CHROMATOGRAPHIC METHOD FOR ANALYSIS OF ANOMERIC CARBOHYDRATES AND FOR DETERMINATION OF MUTAROTATION COEFFICIENTS
A GAS CHROMATOGRAPHIC METHOD FOR ANALYSIS OF ANOMERIC CARBOHYDRATES AND FOR DETERMINATION OF MUTAROTATION COEFFICIENTS
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DOI：
10.1016/0003-2697(67)90036-x
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发表时间：
1967-01-01
影响因子：
2.9
通讯作者：
BOTLOCK, N
中科院分区：
文献类型：
--
作者：
BENTLEY, R;BOTLOCK, N
用二甲基甲酰胺稀释小样本（通常为5 [mu]l），在液态N2温度下冷冻，然后与吡啶、六甲基二氮烷和三甲基氯硅烷的混合物反应。用气相色谱法测定了完全三甲基硅基化碳水化合物的端粒形式的比例。该方法给出了葡萄糖、半乳糖、阿拉伯糖和木糖的突变系数值，与旋光法测定的值相同。本文描述了该方法在酶——诱变酶测定中的应用。
A small sample (usually 5 [mu]l) is diluted with dimethylformamide, frozen at liquid N2 temperature, and then reacted with a mixture of pyridine, hexamethyldisilazane, and trimethylchlorosilane. The proportions of the anomeric forms of the fully trimethylsilylated carbohydrate are determined by gas chromatography. The method gives values for the mutarotation coefficients of glucose, galactose, arabinose, and xylose which are identical to those determined by polarimetry. Application of the method to assay of the enzyme, mutarotase, is described.