T lymphocytes lack rearrangement of the bcr gene in Philadelphia chromosome-positive chronic myelocytic leukemia.
T lymphocytes lack rearrangement of the bcr gene in Philadelphia chromosome-positive chronic myelocytic leukemia.
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费城染色体阳性慢性粒细胞白血病中，T 淋巴细胞缺乏 bcr 基因重排。
DOI：
10.1182/blood.v69.6.1682.bloodjournal6961682
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发表时间：
1987
期刊：
影响因子：
20.3
通讯作者：
P. Bettelheim
中科院分区：
文献类型：
--
作者：
C. Bartram;A. Raghavachar;B. Anger;C. Stain;P. Bettelheim
为探讨T淋巴细胞与Ph阳性慢性粒细胞白血病（CML）的关系，我们分析了12例CML患者T细胞和非T细胞bcr基因的排列。尽管所有患者的非T细胞部分均显示bcr重排，但T细胞群缺乏各自的基因重组。此外，通过使用T细胞受体β链序列的Southern印迹分析，我们的数据表明12例中有11例的T细胞样品具有多克隆性;在一名患者中，可以鉴定出克隆T细胞群体。这些结果表明，大多数Ph阳性CML患者的T细胞系不是来自参与白血病发生的多能干细胞，因此证实了以前的研究基于细胞遗传学或葡萄糖-6-磷酸脱氢酶分析。多克隆T细胞群的证明可能反映了干细胞仅分化为T细胞的持久性。
To study the possible involvement of T lymphocytes in Philadelphia chromosome (Ph)-positive chronic myelocytic leukemia (CML) we analyzed the arrangement of the bcr gene in T cell and non-T cell samples of 12 CML patients. Although all the patients showed bcr rearrangements in non-T cell fractions, T cell populations lacked respective gene recombinations. Moreover, by Southern blot analyses using T cell receptor beta chain sequences our data indicate polyclonality of T cell samples from 11 of 12 cases; in one patient a clonal T cell population could be identified. These results suggest that T lineages of most Ph-positive CML patients are not derived from pluripotent stem cells involved in leukemogenesis and thus confirm previous investigations based on cytogenetic or glucose-6-phosphate dehydrogenase analyses. The demonstration of polyclonal T cell populations may reflect persistence of stem cells committed to differentiate only into T cells.