Genome-wide SNP genotyping reveals hidden population structure of an acroporid species at a subtropical coral island: Implications for coral restoration
Genome-wide SNP genotyping reveals hidden population structure of an acroporid species at a subtropical coral island: Implications for coral restoration
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全基因组 SNP 基因分型揭示了亚热带珊瑚岛顶孔类物种的隐藏种群结构：对珊瑚恢复的影响
DOI：
10.1002/aqc.3626
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Noriyuki Satoh
中科院分区：
文献类型：
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作者：
Yuna Zayasu;Takeshi Takeuchi;Tomofumi Nagata;Megumi Kanai;Manabu Fujie;Mayumi Kawamitsu;Wakana Chinen;Chuya Shinzato;Noriyuki Satoh
对于传统的珊瑚恢复管理和启动新的干预措施以应对全球活珊瑚数量的显著下降来说，必须考虑遗传成分。在实施通过珊瑚恢复实现自给自足生态系统的战略之前，应仔细评估精细空间尺度上的种群遗传结构。从7个采样点采集到140个菌落，从6个采样点采集到81个菌落。总共有384个单核苷酸多态(SNP)基因座。数字化基因和470个SNPs论坛。使用一种相对经济的技术--多重ISSR基因分型--测序获得了Tenue。在这两个顶端物种中，观察到的杂合性显著低于预期的杂合度，这表明遗传多样性不足可能是由于过去大规模的珊瑚漂白造成的。尽管这两个物种都是传播产卵者，但两个物种的种群结构是不同的。未见明显的可检测结构。Digtifera，但在Ina中发现了两个不同的分支。特努伊斯。FA的遗传同质性。库姆岛的DIGITIFERA表明，该物种可以作为该岛遗传分化的重点物种进行积极恢复。相比之下，在一个较小的研究区域内，A.tenuis出人意料地包括了两个不同的支系，很少或没有混合，可能代表了两个繁殖隔离的隐蔽物种。因此，当使用a时。在这个问题得到回答之前，谨慎的做法是避免干扰野生种群的遗传组成。
It is essential to consider genetic composition for both conventional coral restoration management and for initiating new interventions to counter the significant global decline in living corals. Population genetic structure at a fine spatial scale should be carefully evaluated before implementing strategies to achieve self‐sustaining ecosystems via coral restoration.This study investigated the population genetic structure of two acroporid species at Kume Island, Okinawa, Japan. There were 140 colonies ofAcropora digitiferacollected from seven study sites, and 81 colonies ofAcropora tenuisfrom six sites. In total, 384 single nucleotide polymorphism (SNP) loci forA. digitiferaand 470 SNPs forA. tenuiswere obtained using a comparatively economical technique, Multiplexed ISSR Genotyping by sequencing.Observed heterozygosity was significantly lower than expected heterozygosity at all SNP sites in both acroporid species, suggesting deficient genetic diversity possibly caused by past massive coral bleaching. Even though both species are broadcast spawners, the population structure was different in the two species. No detectable structure was evident inA. digitifera, but two distinct clades were found inA. tenuis. The genetic homogeneity ofA. digitiferaat Kume Island suggests that this species could be used as a focal species for active restoration in terms of genetic differentiation at this island. By contrast,A. tenuisunexpectedly included two distinct clades with little or no admixture within a small study area, possibly representing two reproductively isolated cryptic species. Thus, when usingA. tenuis, it would be prudent to avoid disturbing the genetic composition of wild populations until this question is answered.